参仙汤对多发性脑梗死性痴呆大鼠学习记忆能力的干预效应(英文)  

Intervention effect of shenxian tang on the learning and memory power of rats with multiple cerebral infarction dementia

作　　者：仝战旗[1] 赵美玲[1] 郑青平[1] 

机构地区：[1]解放军总医院中医科,北京市100853

出　　处：《中国临床康复》2005年第36期152-154,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:解放军总医院自行研制的参仙汤方,经临床初步观察28例脑血管性痴呆患者,其智能量表分值有明显提高。目的:观察中药复方参仙汤对多发性脑梗死性痴呆大鼠学习记忆的影响,并对其抗自由基损伤能力进行分析。设计:双盲随机对照实验。单位:解放军总医院中医科。材料:实验于1997-05/12在解放军总医院中医科完成。选取3个月龄SD大白鼠72只,随机分为6组:正常对照组,痴呆模型组,阿尼西坦组,参仙汤30,15,7.5g/(kg·d)剂量组,12只/组。参仙汤由人参、仙灵脾、何首乌、地龙等组成。按传统煎药法煎取药液,以恒温水浴锅分别浓缩成含生药2.0,1.0,0.5g/mL。方法:参仙汤30,15,7.5g/(kg·d)剂量组分别按成人每公斤体质量剂量的20,10,5倍给予参仙汤灌胃,剂量分别为生药30,15,7.5g/(kg·d);阿尼西坦组给予100mg/(kg·d)阿尼西坦灌胃;痴呆模型组和正常对照组分别灌以等量生理盐水。连续给药7d,第7天给药后1h开始造模。除正常对照组外,其余各组均造模。左心室注射液体石蜡栓子0.25mL/kg,建立多发性脑梗死模型。造模后继续给药至42d。然后采用水迷宫实验对大鼠学习记忆能力进行评估。实验结束后1h将各组大鼠脱臼处死,进行组织、脏器各项生化指标的测定。主要观察指标:参仙汤对多发性脑梗死性痴呆大鼠水迷宫作业时间、作业正确次数、脏器生化指标的影响。结果:实验纳入72只大鼠,操作过程中脱落9只,最后共63只大鼠进入结果分析。①参仙汤对多发性脑梗死性痴呆大鼠水迷宫作业时间的影响:与痴呆模型组比较,参仙汤15,30g/(kg·d)剂量组于迷宫实验第5天均明显缩短[(24.1±13.3),(14.7±6.9),(13.7±7.5)s,P均<0.05]。②参仙汤对多发性脑梗死性痴呆大鼠水迷宫作业正确次数的影响:与痴呆模型组比较,参仙汤15,30g/(kg·d)剂量组于迷宫实验第5天均显著增加[(1.70±2.07),(3.82±1.89),(4.36±1.75)次,P<0BACKGROUND： Shenxian tang developed in General Hospital of Chinese PLA can obviously raise the intelligence scale through clinical observation of 28 cases of patients with cerebrovascular dementia. OBJECTIVE： To investigate the effect of traditional Chinese drug compound shenxian tang on the learning and memory power of rats with multiple cerebral infarction dementia, and analyze its resisting power to free radical injury. DESIGN： A double-blink and randomized controlled trial. SETTING： Department of Traditional Chinese Medicine of General Hospital of Chinese PLA. MATERIALS： The experiment was completed from May to December 1997 at the Department of Department of Traditional Chinese Medicine of General Hospital of Chinese PLA. A total of 72 SD rats aged 3 months were randomly divided into 6 groups： normal control group, dementia model group, aniracetam group, shenxian tang 30 g/（kg·d）, 15 g/（kg·d） and 7.5 g/（kg·d） groups, with 12 in each group. Shenxian tang consisted of Radix Ginseng, Herba Epimedii. Radix Polygoni Muhiflori, Lumbricus, and so on. According to the traditional decoction making method, the shenxian tang was made and condensed in the thermostat water bath into crude drug contents of 2.0, 1.0, 0.5 g/mL respectively. METHODS： The rats in shenxian tang 30, 15, 7.5 （g/kg·d） groups were by gavage given shenxian tang, respectively in doses 20, 10 and 5 time that for adult person per kilogram body quality, or 30, 15, 7.5 g/（kg·d）. The rats in aniracetam group were by gavage given aniracetam 100 mg/ （kg·d）. Tbe rats in dementia model and normal control groups were by gavage given normal saline of the same volume. The administration was consecutively give for 7 days, one hour after the last administration, the models （except for normal control group） were built up： Liquid paraffin 0.25 mL/kg was injected into the left ventricle of the rat for establishment of multiple cerebral infarction dementia model. After built up of the model, the administration was con

关 键 词：痴呆 多发性梗塞性 迷宫学习 自由基 中药疗法 汤剂 

分 类 号：R743[医药卫生—神经病学与精神病学]