参麻益智胶囊对血管性痴呆大鼠体内过氧化状态的影响(英文)  

Effects of Cenma Yizhi Capsule on peroxidative status in vivo in rats with vascular dementia

作　　者：宋光熠[1] 王晓颖[1] 段有金[1] 李开明[1] 

机构地区：[1]辽宁省基础医学研究所药理研究室,辽宁省沈阳市110101

出　　处：《中国临床康复》2005年第36期160-162,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:血管性痴呆的发生和发展与氧自由基代谢的异常关系密切,在痴呆病的药效学实验中常以氧自由基的代谢作为主要指标。目的:建立多发性脑梗死动物模型,测定大鼠血液与脑组织内的脂质过氧化物含量、超氧化物歧化酶及谷胱甘肽过氧化物酶的活性,从而分析参麻益智胶囊对血管性痴呆大鼠体内过氧化状态的影响。设计:随机对照单一样本实验。单位:辽宁省基础医学研究所药理研究室。材料:实验于1997-07/1998-11在辽宁省基础医学研究所药理研究室完成。选取健康Wistar大鼠96只,随机分为6组,即正常对照组、模型对照组、阳性药对照组、参麻益智胶囊高剂量组、参麻益智胶囊中剂量组、参麻益智胶囊低剂量组,16只/组。参麻益智胶囊由人参、天麻等中药总提取物组成,每克药粉含生药2.7g,由中国中医研究院西苑医院提供。方法:除正常对照组外,其余各组均建立多发性脑梗死动物模型。正常对照组及模型对照组均灌胃给予生理盐水0.5mL;参麻益智胶囊高、中、低剂量组分别灌胃给药3.2,1.6,0.8g/kg;阳性药对照组灌胃给予双氢麦角碱1mg/Kg。各组于栓塞后1周开始给药,1次/d,连续6周。脂质过氧化物含量按荧光法测定,超氧化物歧化酶活性应用邻苯三酚自氧化法测定,谷胱甘肽过氧化物酶活性按Hafenam方法测定。主要观察指标:各组大鼠血浆与脑组织中脂质过氧化物含量、谷胱甘肽过氧化物酶和超氧化物歧化酶活性的测定。结果:实验纳入96只大鼠全部进入结果分析。①各组大鼠血浆中各项生化指标测定结果:脂质过氧化物含量模型对照组及阳性药对照组明显高于正常对照组[(16.0±1.6),(16.0±1.7),(13.4±1.0)μmol/L,t=4.20～10.58,P均<0.01],参麻益智高、中、低剂量组显著高于模型对照组[(13.1±1.5),(13.3±0.8),(13.9±1.0),(16.0±1.6)μmol/L,t=4.39～11.69,P均<0.01];与正常对照组谷胱甘肽过�BACKGROUND： The incidence and progress of vascular dementia （VD） is closely related to the abnormal metabolism of oxygen-derived free radicals, whose metabolism is often the main index in pharmacodynamic experiments on dementia. OBJECTIVE： To establish the animal model of multiple cerebral infarction and determine the content of lipid peroxide and the activity of superoxide dismutase and glutathione peroxidase in the blood and cerebral tissues so as to analyze the effects of Cenma Yizhi Capsule on the oxidative status in vivo in VD rats. DESIGN： Randomized controlled single sample trial. SETTING： Department of Pharmacology, Institute of Basic Medicine of Liaoning Province. MATERIALS： This experiment was conducted in the Department of Pharmacology, Institute of Basic Medicine of Liaoning Province, between July 1997 and November 1998. Totally 96 healthy Wistar rats were recruited and randomized into 6 groups： normal control group, model control group , positive drug control group, Cenma Yizhi Capsule high-dose group, Cenma Yizhi Capsule medium-dose grgup, and Cennut Yizhi Capsule low-dose group with 16 rats in each group. Cenma Yizhi Capsules, the compound of the total extract of ginseng, Tiannia and other Chinese herbal medicine （each gram contained 2.7 g drug）, were provided by Xiyuan Hospital of the Chinese Academy of Traditional Chinese Medicine. METHODS： The animal model of multiple cerebral infarction was established in all the groups except normal control group. Normal saline of 0.5 mL was gastrically perfused into the rats in Cenma Yizhi Capsule high-, medium- and low-dose groups; dihydroergotamine mesylate of 1mg/kg was gastrieally perfused into the rats in positive drug control group. Rats in each group were given the drug 1 week after embolism, once a day for 6 consecutive weeks. The content of lipid peroxide was determined with fluorescence method, and the activity of superoxide dismutase and glutathione peroxidase was determined with self-oxidation method of pyrogallol and Hafenam

关 键 词：痴呆 血管性/中药疗法 过氧化脂质类 谷胱甘肽过氧化酶 超氧化物歧化酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]