痴呆模型鼠脑海马突触素改变及其与学习记忆的关系(英文)  

Changes of hippocampal synaptophysin in rat model of dementia and the relationship of the changes with learning and memory

作　　者：洪乐鹏[1] 龙大宏[1] 冷水龙[1] 

机构地区：[1]广州医学院人体解剖学教研室,广东省广州市510182

出　　处：《中国临床康复》2005年第36期176-178,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省医学科研基金资助(No:A2000252)~~

摘　　要：背景:学习记忆与中枢神经系统突触的结构和功能的可塑性密切相关。目的:探讨痴呆模型鼠海马的突触素改变及其与学习记忆的关系,为老年性痴呆的发病过程提供实验依据。设计:随机对照实验。单位:广州医学院人体解剖学教研室。材料:实验于2003-02/2004-05在广州医学院人体解剖学教研室完成。选取成年SD大鼠20只,随机分为正常组和损伤组,10只/组。方法:损伤组大鼠切断左侧穹窿海马伞,建立基底前脑-海马胆碱能通路受损的痴呆模型。造模4周后,用三等分Y型迷宫检测两组大鼠的学习记忆能力。大鼠学习成绩以连续10次测试中有9次达到正确反应(2min以内逃避电击并第一时间到达安全区)时所需的训练次数来表示,记录每只大鼠达到学会标准所需的训练次数,训练次数少说明学习能力强。训练结束24h后测试记忆能力,以10次训练中的正确反应次数代表记忆保持能力的优劣,正确反应次数少则记忆能力低。然后用免疫组织化学染色和图像分析技术等方法对大鼠海马突触素进行定量分析,以实际吸光度值进行比较,以避免染色过程中的非特异性染色导致的误差。主要观察指标:①两组大鼠行为测试中学会逃避电击并第一时间到达安全区所需的训练次数。②两组大鼠海马结构突触素免疫反应物检测结果。③两组大鼠损伤侧海马CA1区和齿状回分子层突触素免疫反应物的实际吸光度值。结果:实验纳入大鼠20只,全部进入结果分析。①两组大鼠学会逃避电击并第一时间到达安全区所需的训练次数:损伤组学习能力达到标准所需的训练次数明显多于正常组(98.40±4.51),(62.21±2.43)次,P<0.01;而记忆能力达到标准所需的训练次数则明显低于正常组(3.82±0.64),(8.81±0.32)次,P<0.01。②两组大鼠海马结构突触素免疫反应物检测结果:海马结构突触素免疫反应物呈板层分布,神经元胞体、BACKGROUND： Learning and memory are closely relevant to the plasticity of structure and function of synapse in central nervous system. OBJECTIVE： To investigate the changes of hippocampal synaptophysin in rat model of dementia and the relationship of the changes with learning and memory so as to provide experimental evidences for the pathogenic course of senile dementia. DESIGN： Randomized controlled trial. SETTING： Teaching ＆ Research Section of Anthropotomy, Guangzhou Medical College. MATERIALS： The experiment was completed in Teaching ＆ Research Section of Anthropotomy of Guangzhou Medical College from February 2003 to May 2004. A total of 20 aduh SD rats were selected and randomly divided into normal group＇and injury group with 10 in each group. METHODS： Left fimbria-fornix transection was performed on rats in injury group in order to establish rat models of dementia with fundus forebrain-damaged hippocampal cholinergic pathway. Four weeks later, detection of learning and memory ability was carried out on rats in each group with trisection Y-maze. Learning results was shown by training frequency of 9 out of 10 times of continuously correct responds （escaping to safe area after electric shock at once in 2 minutes）. Frequencies of responses required reaching the standard were recorded. Low frequency indicated good learning ability. The detection was repeated after 24 hours to observe memory. Memory was shown by correct response rate out of 10 times of training. Low rate indicated poor memory. After that, quantitative analysis was made on hippocampal synaptophysin of rats with the method of immunohistochemical staining and image analysis technique. Comparison of the actual absorbance value was conducted in order to eliminate the errors caused by non-specific staining during the process of staining. MAIN OUTCOME MEASURES： ① The training frequencies acquired to escape to safe area after electric shock at once in behavior test. ② The detected results of immnoreactant of hippocampal synap

关 键 词：痴呆 记忆 海马 突触 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]