脑梗死大鼠运动训练后星形胶质细胞与突触和运动功能的变化  

Effect of mobility training on astrocyte, synapse and motor skill in rats with acute cerebral infarction

作　　者：谭来勋[1] 孙圣刚[2] 张双国[1] 吴旭[1] 管强[2] 

机构地区：[1]武汉大学人民医院神经科,武汉430060 [2]华中科技大学同济医学院附属协和医院神经科

出　　处：《中华物理医学与康复杂志》2005年第10期581-584,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家教育部高等学校博士点学科专项科研基金(No.20020487065)

摘　　要：目的研究脑梗死大鼠运动训练后梗死周边区星形胶质细胞、突触和运动功能的变化及相关性。方法将120只大脑中动脉闭塞脑梗死模型大鼠随机分为康复组、抑制组、盐水组和对照组,以横木行走试验评定运动功能,在7,21,42d应用电镜和免疫组织化学方法,观察各组大鼠脑梗死周边区星形胶质细胞、突触、胶质纤维酸性蛋白和突触素等的变化。结果康复组大鼠3周末的运动功能评分为(3.2±0.3)分,6周末为(5.8±0.9)分,对照组3周末为(1.8±0.5)分,6周末为(2.6±0.8)分;抑制组3周末为(1.6±0.9)分,6周末为(2.1±0.7)分。康复组比对照组和抑制组高,P<0.01。在8000倍电镜视野下,康复组3周末脑梗死周边区星形胶质细胞为(5.8±0.3)个,6周末为(8.5±0.8)个;3周末突触数目为(12.3±1.3)个,6周末为(16.3±1.4)个。星形胶质细胞和突触数目呈同步增多,两者均明显多于对照组和抑制组(P<0.01)。免疫组化染色观察显示,康复组脑梗死周边区胶质纤维酸性蛋白和突触素阳性信号密度值也在3,6周末高于对照组和抑制组(P<0.01)。结论脑梗死大鼠运动训练可促使星形胶质细胞增殖,加强突触重建和功能修复,促进运动功能恢复;星形胶质细胞增殖可能在其中发挥关键性作用。Objective To investigate the change of and relationship between astrocytes and synapse structure in the region around the infarction area and the roles of the astrocytes on the recovery of motor skill in the experimental rats with acute cerebral infarction after mobility training. Methods A total of 120 rats were randomly divided into 4 groups： a rehabilitation group, an inhibition group, a saline group and a control group. The motor skill of the rats was examined by beam walking test. The changes of astrocytes, synapses, the glial fibrillary acidic protein （GFAP） , synaptophysin and growth associated protein 43 （ GAP-43 ） in the region around the infarction were observed using electron microscope and immunohistochemistry staining at 7,21 and 42 d after the model was made. Results It was noted that the recovery of motor skill was more significant in the rehabilitation group than that in the control and the inhibition groups （ scored in beam walking test at 3.2 ± 0.3 vs 1.8 ±0.5 and 1.6 ±0.9 at the third weekend; scored 5.8 ±0.9 vs 2.6 ±0.8 and 2. 1 ± 0.7scores at the sixth weekend. P 〈 0.01 ）. It was observed under the electron microscope that the number of astrocyte increased gradually （from 5.8 ±0.3 / visual field at the 3rd weekend to 8.5 ± 0.8 / visual field at the 6th weekend） , accompanying the simultaneous increase of synapse number （12. 3 ± 1.3/visual field at the 3rd weekend to 16.3 ± 1.4 /visual field at the 6th weekend）over time, and the increase of the number of astrocyte was also more significant in the rehabilitation group than that in the control and the inhibition groups（ P 〈 0.01 ）. The immunohistochemistry staining showed that the optical density of GFAP and synaptophysin in the region around the infarction area of the rehabilitation group was significantly higher than those in the control and the inhibition groups at both the 3rd and the 6th weekend（P 〈0.01 ）. Conclusion The motor skill training could promote the increase of astrocyte number and th

关 键 词：星形胶质细胞 突触可塑性 康复 脑梗死 运动功能评分 模型大鼠 突触素 训练后 胶质纤维酸性蛋白 免疫组织化学方法 

分 类 号：R743.33[医药卫生—神经病学与精神病学]