小麦高分子量麦谷蛋白亚基1Dx5启动子驱动外源基因的表达  

Expression of Foreign Genes in Wheat (Triticum aestivum L.) Drived by HMW- GS 1Dx5 Promoter

作　　者：姚琴[1] 丛玲[1] 罗立廷[1] 李三和[1] 何光源[1] 

机构地区：[1]华中科技大学生命科学与技术学院,中英HUST-RRes基因工程与基因组学联合实验室,武汉430074

出　　处：《植物生理学通讯》2005年第5期583-586,共4页Plant Physiology Communications

基　　金：国家高新技术项目"863"计划(2002AA224031)

摘　　要：将小麦高分子量麦谷蛋白亚基(HMW-GS)基因的胚乳组织特异性表达启动子驱动的外源突变型1Dx5基因和gus基因导入小麦中。对其转基因植株连续3代的跟踪研究表明,突变型1Dx5基因的重复序列导致其表达蛋白分子量增大,并影响其它1Bx17+1By18亚基基因的表达。组织化学分析观察到gus基因在1Dx5基因启动子驱动下的表达表现出胚乳组织特异性,在开花2周后开始表达,表达量呈持续上升,至腊熟期达到最高,其次为籽粒成熟期。The foreign genes including gus and 1Dx5 mutant engineered to possess more of the repetitive central domain drived by HMW-GS （high molecular weight glutenin subunits） promoter were introduced into wheat, which allows obtaining the stable expression transplant. The consecutive investigation in 3 progenies indicated the repetitive sequence of 1Dx5 mutant improved its molecular weight and influenced expression of 1Bx17＋ 1By18. To test tissue specificity, the gus tests in seeds of transgenic plants exhibited positively, but negatively in leaves, roots, anthers or pollens. Foreign gus gene began to express two weeks after flowering, and expression levels kept increased trend to waxy ripening stage.

关 键 词：突变型 高分子量麦谷蛋白亚基 特异性表达 基因启动子 外源基因 驱动 小麦 组织特异性表达 Dx5基因 GUS基因 

分 类 号：S512.1[农业科学—作物学]