钯(Ⅱ)三元配合物稳定性及其与DNA作用研究  

Studies on Stability of Pd(Ⅱ)Ternary Complexes and Interaction with DNA

作　　者：高恩君[1] 丁丽娜[1] 刘祁涛[2] 孙亚光[1] 

机构地区：[1]沈阳化工学院化工系,沈阳110142 [2]辽宁大学化学科学与工程学院,沈阳110036

出　　处：《物理化学学报》2005年第10期1091-1095,共5页Acta Physico-Chimica Sinica

基　　金：辽宁省教育厅基金(2004c021);沈阳化工学院博士基金;拔尖人才基金资助项目~~

摘　　要：合成了[Pd(bipy)(DL-gly)]Cl·2H2O(1)和[Pd(bipy)(L-lys)]C·lH2O(2)两个钯三元配合物.配合物1和2的稳定常数对数值lgβ分别为13.81和13.71,表征常数驻lgK、lgX高于统计值,表明在配合物分子内存在d-pπ电子协同效应.紫外光谱、荧光光谱结果表明,配合物1和2与鱼精DNA主要以插入方式键合,键合常数分别为2.37×106(1)和4.57×106(2).CD光谱图也显示,配合物与DNA分子之间发生了作用.质粒pBR322DNA的凝胶电泳图表明,配合物浓度在3.00×10-3～7.50×10-4mol·L-1范围内对DNA分子有切割作用,配合物浓度低于3.75×10-4mol·L-1时则主要以插入方式与DNA键合.Two ternary complexes [Pd（bipy）（DL-gly）]Cl· 2H2O（1） and [Pd（bipy）（L-lys）]Cl·H2O（2） were synthesized. The stability constants lgfl of two complexes are 13.81（1） and 13.71（2）, respectively, and relative stability AlgK, lgX are higher than statistical value. The results show that there exists d-p π electronic effect in complexes. Intercalation of the complexes with DNA can be demonstrated by UV-Vis spectra and fluorescence spectra.The binding constans of the two complexes with sperm DNA are 2.37×10^6（1） and 4.57×10^6（2）, respectively. It shows that the interaction intensity is 1〈2.The CD spectra also show that the tow complexes were reacted with DNA. The results of gel electrophoresis experiments reveal that plasmid pBR322 DNA was cleaved by the two complexes when complex concentration ranges from 3.00×10^-3 to 7.50×10^-4 mol·L^-1, whereas insertion is main binding model when complex concentration is lower than 3.75×10^-4 mol· L^-1.

关 键 词：钯(Ⅱ)三元配合物 稳定性 联吡啶 氨基酸 DNA 插入作用 

分 类 号：O641.4[理学—物理化学]