烟草木质素合成途径几个中间代谢物HPLC分析  

HPLC analysis of metabolites in the biosynthetic pathway of tabacco lignin.

作　　者：陶霞娟[1] 赵艳玲[1] 陈雪梅[1] 王天华[1] 蒋湘宁[1] 

机构地区：[1]北京林业大学生物科学与技术学院

出　　处：《北京林业大学学报》2005年第5期111-114,共4页Journal of Beijing Forestry University

基　　金：国家自然科学基金(30271066);"973"国家重大基础研究规划项目(G1999016005).

摘　　要：该文建立了烟草木质素生物合成途径几个中间代谢物咖啡酸(CA)、4香豆酸(PA)和阿魏酸(FA)的RPHPLC分析方法.用ODSC18色谱柱分离、紫外波长扫描检测器检测,并确定320nm为最佳检测波长;V(甲醇)∶V(水)(含1.5%乙酸)为20∶80,是最理想的流动相.该方法对CA、PA和FA的回收率分别为95.59%、93.67%、97.32%,相关系数(R2)分别为0.9946、0.9946、0.9945,满足定量分析要求.经测定,发现转基因烟草CA、PA和FA含量分别为未转化烟草的2.6～3.0、3.4～4.1和7.1～8.2倍.A method of RP-HPLC with a ODS-C18 column and a UV detector was developed to separate and detect three metabolites such as the caffic acid （CA）, 4-conmaric acid （PA） and ferulic acid （FA）, in the biosynthetic pathway of tobacco lignin. The optimum separation conditions were suggested with the mobile phase containing 1.5% acetic acid （ V （methanol） ： V（water） = 20：80） and flow rate 1.0 mL/min and the UV detection wavelength of 320 nm. The recovery rates of CA, PA and FA were 95.59% ,93.67% and 97.32%, and the linear correlation coefficients were 0. 994 6, 0. 994 6 and 0. 994 5 respectively. The results showed that the method of RP-HPLC was suitable for quantitative analysis of the three metabolites. The contents of CA, PA and FA in transgenic tobacco plants were 2.6-3.0,3.4-4.1 and 7.1-8.2 fold higher than those in wild type tobacco plants,

关 键 词：烟草 木质素 RP-HPLC 咖啡酸 4-香豆酸 阿魏酸 

分 类 号：TQ351.377[化学工程] S572[农业科学—烟草工业]