中药多糖在信号转导中诱导细胞凋亡的探讨  

Study of the Cell Apoptosis and Mechanism of Plant Polysaccharide in Cellular Signalling Pathway

作　　者：冯刚[1] 罗利琼[2] 孔庆志[1] 黄涛 卢宏达[3] 

机构地区：[1]武汉市普爱医院,430033 [2]湖北省中医学院 [3]武汉市卫生局

出　　处：《肿瘤防治研究》2005年第9期557-559,共3页Cancer Research on Prevention and Treatment

摘　　要：目的研究中药多糖在信号转导中诱导细胞凋亡的作用并探讨其作用机制。方法培养1、8h后,经流式细胞仪检测细胞凋亡情况。结果茯苓多糖、黄芪多糖、猪苓多糖诱导S-180肉瘤细胞的凋亡,其效果与茯苓多糖、黄芪多糖、猪苓多糖的浓度和作用时间有关。实验组培养液,分别加入茯苓多糖、黄芪多糖、猪苓多糖各100μg/mL、250μg/mL和500μg/mL,培养1、8h后细胞凋亡率有增高趋势。实验组细胞凋亡率与对照组相比有显著性差异(P<0.05)。结论中药多糖在早期能诱导细胞凋亡,在晚期能引起细胞坏死,抑制S-180肉瘤细胞的增殖。Objective To study the cell apoptosis and probe its under lying mechanism of plant polysaccharide in cellular signalling pathway. Methods After cultured for 1 and 8h, the cell apoptotic rate of mice sarcoma S180 cell line was observed by flow cytometry. Results cell apoptosis of mice sarcoma S180 cell line was induced by PB （ polyporusum bellatus） PPS（ pachyman polysaccharides） APS（astragalus polysaccharides） in dose-and time-dependent manners. After respectably treated with 100,250,500ptg/mL of Plant polysaccharide for 1 and 8h. The cell apoptotic rate of mice sarcoma S180 cell line were on the rise with time and dose. The apoptotic rate of experiment groups have significant difference compared with control （P〈0. 05）. Conclusion Plant polysaccharide may induce cell apoptosis of S180 cell line in early phase and induce necrocytosis in late phase,inbihits proliferation of mice sarcoma S180 cell.

关 键 词：中药多糖 信号转导 肿瘤 细胞凋亡 流式细胞仪 

分 类 号：R73-34[医药卫生—肿瘤]