儿茶素通过ERK抑制兔晶状体上皮细胞增殖  

Decreased proliferation of rabbit lens epithelial cells induced by EGCG depending on the inhibition of MEK activation

作　　者：黄文勇[1] 曾骏文[1] 刘奕志[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《眼科研究》2005年第5期501-503,共3页Chinese Ophthalmic Research

基　　金：广东省科技计划项目基金资助(20030087)

摘　　要：目的探讨绿茶提取物表没食子儿茶素没食子酸酯(EGCG)抑制兔晶状体上皮细胞增殖时,细胞外信号调节激酶(ERK)信号转导通路所起的作用。方法用组织块培养法获取新西兰白兔晶状体上皮细胞,用噻唑蓝比色法(MTT)研究晶状体上皮细胞增殖作用;用Westernblot法研究ERK的磷酸化和非磷酸化水平。结果预先分别加入25、50μmol/L的ERK特异性抑制剂PD980059孵育1h后,50、100、200μmol/LEGCG对晶状体上皮细胞的抑制率明显大于对照组(P<0.05)。在晶状体上皮细胞,磷酸化的ERK基础水平很高;200μmol/LEGCG组ERK的磷酸化水平最高,随EGCG浓度降低其水平下降;加入EGCG后早期,ERK的磷酸化水平最高,随时间的延长仍保持高水平。各组的非磷酸化ERK水平始终保持不变。结论EGCG可能通过调控ERK的磷酸化水平而抑制晶状体上皮细胞的增殖,并不影响总蛋白浓度。Objective To explore the role of mitogen-aetivated protein kinase-extracellular signal-regulated kinase （ MEK- ERK） pathway in the growth inhibition of rabbit lens epithelial cells （LECs） induced by （-）-epigallocateehin-3-gallate （EGCG）. Methods LECs were obtained from New Zealand white rabbit. MTT colormetric assay was used to evaluate the cell prolifration with or without 50,100 and 200 μl/L addition in the cellmedium. Western blotting was used to study the kinsae phosphorylatied- and nonphosphorylatied-levels of ERK. Results （ 1 ） When LECs were preincubated with 25,50 i.rmol/L PD980059 for 1 hours, the inhibition of the cell proliferation in the concentration of 50, 100 and 200 μmol/L EGCG was significantly increased in compared to that without PD980059 （ P 〈 0. 05 ）. （ 2 ） Both the basic-levels of phosphorylatied- and nonphosphorylated-of ERK in LECs were higher and the highest ERK activation was demonstrated in the cells with addition of 200 I.rmol/L EGCG, however, it was reduced with the decrease of EGCG concentration. 15 minuts after EGCG was added, the phosphorylatied-level reached its peak. The nonphosphorylatied- level of ERK remained constant through the testing period. Conclusion EGCG inhibits LECs proliferation by regulating ERK phosphorylatied-level.

关 键 词：表没食子儿茶素没食子酸酯 细胞外信号调节激酶 晶状体上皮细胞 

分 类 号：R285[医药卫生—中药学]