瑞香狼毒素对菜粉蝶幼虫的杀虫活性  

The toxicity of the extract from the root of Stellera chamaejasme against Pieris rapae

作　　者：张可君[1] 何义国[1] 周俗[2] 肖波[1] 侯太平[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川省草原工作总站,成都610041

出　　处：《植物保护学报》2005年第3期305-308,共4页Journal of Plant Protection

基　　金：教育部新世纪优秀人才计划(NCET-04-0868);四川省应用基础项目(01NY051-02)

摘　　要：在本实验室前期工作中,发现生长于青藏高原的有毒植物瑞香狼毒对菜青虫具有较好的防治效果。采用微量点滴法研究瑞香狼毒素对菜青虫的触杀活性,以叶片夹毒法和灌喂法研究胃毒活性。在触杀活性试验中,72h 的致死中量(LD_(50))为0.96μg/头,以6.25×10~3 mg·L^(-1)浓度的样品液2μL 点滴试虫,即1.25×10μg/头处理组的72h 校正死亡率为92.2%。在胃毒活性试验中,72h 叶片夹毒法的致死中浓度(LC_(50))为3.82×10~3mg·L^(-1),5.00×10~4 mg·L^(-1)浓度处理组校正死亡率为91.2%;72h 灌喂法的 LD_(50)为3.80μg/头,以5.00×10~4mg·L^(-1)浓度的样品液2μL 灌喂试虫,即1.00×10~2μg/头的处理组的72h 校正死亡率为96.8%。结果表明,瑞香狼毒素对菜青虫具有较好的触杀活性和一定的胃毒活性。The contact toxicity and stomach toxicity of extract from the root of Stellera chamaejasme against the larvae of Pieris rapae was tested. The experimentation on contact toxicity was conducted with micro topical application. In 72h, the median lethal dose （LD50） is 0.96 μg/larvae and the corrected mortality of the larvae treated with 1.25 x 10μg/larvae is 92.2%. The experimentation on stomach toxicity was conducted by leaf-sandwich and mouthparts infusion. In 72h, the median lethal concentration （LC50） of the larvae treated by leaf-sandwich is 3.82× 10^3 mg·L^-1 and the correct mortality of the larvae treated with 5.00 × 10^4μg/larvae is 91.2%. LD50 of the larvae treated with mouthparts infusion is 3.80μg/larvae and the correct mortality of the larvae treated with 1.00 × 10^2 μg/larvae is 96.8%. The toxicity of the extract mentioned above showed well contact toxicity and some stomach toxicity.

关 键 词：瑞香狼毒 菜粉蝶 触杀活性 胃毒活性 菜粉蝶幼虫 杀虫活性 毒素 致死中浓度 校正死亡率 活性试验 

分 类 号：S482.3[农业科学—农药学] S436.3[农业科学—植物保护]