九节龙皂苷Ⅰ对环磷酰胺模型小鼠免疫功能的影响  

Effect of ardipusilloside-Ⅰ on immunological function in immunosuppressed mice induced by cyclophosphamide

作　　者：曹永孝[1] 郑建普[1] 陶小军[1] 刘静[1] 龙静雯[1] 

机构地区：[1]西安交通大学医学院药理学系环境与疾病相关基因教育部重点实验室,陕西西安710061

出　　处：《中国药学杂志》2005年第20期1553-1555,1571,共4页Chinese Pharmaceutical Journal

摘　　要：目的研究九节龙皂苷Ⅰ(AR-Ⅰ)对环磷酰胺(CTX)模型小鼠免疫功能的影响。方法以CTX(100 mg·kg^(-1))制造小鼠免疫低下模型,同时灌胃给AR-Ⅰ8 d,测定小鼠巨噬细胞吞噬率、血清50%溶血值、自然杀伤细胞和淋巴因子激活的杀伤细胞活性以及小鼠血清IL-2,TNF-α和TFN-γ含量,并观察二硝基氟苯诱导的小鼠迟发性超敏反应。结果AR-Ⅰ可增强CTX所致免疫低下小鼠巨噬细胞吞噬功能和迟发性超敏反应,促进血清溶血素形成,提高自然杀伤细胞和淋巴因子激活的杀伤细胞的杀伤活性,增加血清细胞因子IL-2,TNF-α和IFN-γ的含量。结论AR-Ⅰ能明显改善CTX抑制的小鼠免疫功能,这可能是其抗肿瘤活性的作用机制之一。OBJECTIVE To study the immunomodulation of ardipusilloside- Ⅰ （AR- Ⅰ ） on immunosuppressed mice induced by cyelophosphamide （CTX）. METHODCS The immunosuppressed miee indueed by CTX were administrated with AR- Ⅰ 25 ～ 100 mg· kg^- 1 for 8 d. The phagocytosis ability of macrophage, 50% serum hemolysin concentration（HC50）, the activities of natural killer cells and lymphocyteactivated killer cells, and the level of IL-2, TNF-α and IFN-γ products in serum were measured. The delayed type hypersensitivity （UTH） was also observed. RESULTS AR- Ⅰ （ 25,50 and 100 mg· kg ^- 1 , ig for 8 d） strengthened the macrophage phagoeytosis and DTH, promoted the serum HC50, increased NK cell and LAK cell activities and the produets of IL-2, TNF-α and IFN-γ in serum signifieandy. CONCLUSION AR-Ⅰ can improve immunological function inhibited by CTX in miee, which may be an important antitumor mechanism of AR- Ⅰ.

关 键 词：九节龙皂苷 免疫功能 迟发性超敏反应 自然杀伤细胞 淋巴因子激活的杀伤细胞 细胞因子 

分 类 号：R967[医药卫生—药理学]