短段木灵芝优质高产菌株“赤芝6号”研究  

Study on“Red Reishi No.6”,a high-yield and fine-quality strain of Ganoderma lucidum for woodlqg cultivation

作　　者：李开本[1] 陈体强[1] 何修金 陈世濂[1] 郑宇[1] 朱培根[1] 

机构地区：[1]福建省农科院植物保护研究所

出　　处：《福建省农科院学报》1996年第3期1-6,共6页

基　　金：省科委计划项目

摘　　要：描述了“赤芝６号”主要栽培性状与商品性状。采用光学显微镜和扫描电镜观察了担孢子，结果表明：孢子多呈卵圆形，顶端平截或钝圆锥形，大小为６．５３～８．０４μｍ×９．５５～１２．５６μｍ，孢子表面明显可见一些微小的凹陷。采用扫描电镜观察了菌肉菌丝：其骨架菌丝的骨架干直径２．６～３．９５μｍ，分枝直径１．８～２．６μｍ；缠绕菌丝大多弯曲，直径１．１～１．５μｍ。利用聚丙烯酰胺梯度浓度凝胶电泳法分析比较了“赤芝６号”与福建其他灵芝菌株的酯酶同工酶，结果表明：“赤芝６号”有１５条酶带分布在凝胶浓度８．９％～２５．６％处，其酶谱特征与其他菌株明显区别。Red Relshi No.6”with high-yield and fine-quality for woodiog cultivation was princi-pally applied and popularized by our institute in Fujian province.In this paper,the log-cultivated and economic characters were described.Electric scanning microscopy(ESM)and light rnicroscopy were used to study the basldiospore.The results showed that most of spores were ovate-obrotund with trancated or abtuse apex,their size were 6.53～8.04×9.55～12.56μ m,some shallow minute hollows were evident on the spore surface.Under ESM,the trunk of skelete hyphae had a diameter of 2.6～3.95μm and the branch had a diameter of 1.8～2. 6μ m,the majority of binding hyphae were tortuous and had a diarneter of 1.1～1.5μm。 Easteraes zymograms of“Red Reishi No.6”and other strains in Fujian province,were analysed and compared by gradient concentration polyacry-lamide gel electrophoresis(GC-PAGE).The resuits showed that“Red Reishi No.6”had 15 bands ditributed in gelconcentration ranging from 8.9%to 25.6%,with remarkable difference from oth-er strins.

关 键 词：灵芝菌株 段木 栽培 赤芝6号 

分 类 号：S567.310.4[农业科学—中草药栽培]