结肠癌组织中p53和cyclinD1基因的表达检测及其临床意义  

The Expressions and Its Clinical Significance of p53 and CyclinD1 Genes in Human Colon Cancer

作　　者：郭健政[1] 林茂松[2] 陈卫昌[2] 张宝峰[3] 方静[3] 周琼[3] 胡莺[3] 郜恒骏[3] 

机构地区：[1]苏州大学附属第一医院老年医学科 [2]苏州大学附属第一医院消化内科,江苏苏州215006 [3]生物芯片上海国家工程研究中心上海芯超生物科技有限公司,上海201203

出　　处：《苏州大学学报（医学版）》2005年第5期842-845,共4页Suzhou University Journal of Medical Science

基　　金：国家863计划功能基因组与生物芯片重大专项资助课题(2002AA2Z2021);江苏省"六大人才高峰"资助课题(D类项目)

摘　　要：目的研究结肠癌、腺瘤和正常肠粘膜组织中p53和cyclinD1基因的表达及其临床意义。方法取85例结肠癌、18例结肠腺瘤和9例癌旁正常结肠粘膜组织分别制成72点和104点两块组织芯片,采用免疫组织化学法检测结肠组织芯片中p53和cyclinD1的表达。结果p53、cyclinD1在结肠癌、结肠腺瘤和正常结肠粘膜组织中的表达差异显著(P=0.003,P=0.000),p53c、yclinD1在结肠癌中的表达高于结肠腺瘤和正常结肠粘膜组织,不同组织学分化程度的结肠癌中p53的表达差异显著(P=0.005),cyclinD1的表达与结肠癌临床分期有关(P=0.038),与其他临床病理资料无关。结论p53畸变可能是结肠癌发生的较晚期事件,cyclinD1高表达可与大肠癌发生有关,cyclinD1高表达的肿瘤更易浸润和发展。Objective To study on the expressions and clinical significance of p53 and cyclinD1 in colon cancer, adenomatous polyps and normal para-cancer colon mucosa. Methods Eighty five cases of colon cancer, 18 cases of adenomas and 9 cases of para-mucosa were accumulated and made into two tissue microarrays which containing 72 dost and 104 dots respectively. Expressions of p53 and cyclinD1 proteins were detected by immunohistocheical staining to these microarrays. Results Significant differences were found among colon cancer, adenomas and benign para-cancer mucosa according to the expressions of p53 and cyclinDl（P = 0. 003, P = 0. 000）. Expressions of p53 and cyclinD1 in cancer were highter than that in adenomatous polyps and normal para-cancer colon mucosa. A significant difference of expressions of p53 in different histological grades（P = 0. 005） was found in colon cancer. There was no correlation of expression of cyclinD1 with clinicopathological data but clinical stages （P = 0. 038） in colon cancer was found. Conclusions Over-expression of p53 may be a late event involving in the development of colon cancer an high expressions of cyclinD1 may correlate with the tumorgenesis in colon and may facilitate invasion and progression.

关 键 词：结肠癌 细胞周期 P53 CYCLIND1 免疫组织化学 组织芯片 CYCLIND1基因 结肠癌组织 临床意义 表达检测 

分 类 号：R737.902[医药卫生—肿瘤] R735.35[医药卫生—临床医学]