Chemical Modification of Tryptophan Residues in Pullulanase  

作　　者：Li Rong TENG Hao FAN Yuan Yuan ZHANG Qi YU Yue Feng HUANG Lan Ying LIU 

机构地区：[1]College of Life Science, Jilin University, Changchun 130023

出　　处：《Chinese Chemical Letters》2005年第10期1335-1336,共2页中国化学快报（英文版）

摘　　要：Tryptophan（Trp）residues in pullulanase have been chemically modified with N-bromossuccinimide（NBS）. The results of ultraviolet spectra indicated that there are 18 Trp residues in pullulanase and nine of them are located on the surface of the enzyme. Three of these Trp residues are none-essential residues which showed the fastest reaction speed by Zhou＇s plot. Two of the seven relative faster reacting residues are essential for the activity of the enzyme. The other eight are none-reactive residues with lowest reaction speed.Tryptophan（Trp）residues in pullulanase have been chemically modified with N-bromossuccinimide（NBS）. The results of ultraviolet spectra indicated that there are 18 Trp residues in pullulanase and nine of them are located on the surface of the enzyme. Three of these Trp residues are none-essential residues which showed the fastest reaction speed by Zhou＇s plot. Two of the seven relative faster reacting residues are essential for the activity of the enzyme. The other eight are none-reactive residues with lowest reaction speed.

关 键 词：PULLULANASE tryptophan（Trp） chemical modification. 

分 类 号：O629.8[理学—有机化学]