锂-匹罗卡品致癫大鼠早期大脑皮质MyT1 mRNA表达变化  

Early Phase Expression of MyT1 mRNA in Brain Cortex of Epileptic Rats Induced by Chloride Lithium-pilocarpine

作　　者：吴莹[1] 李华杰[1] 

机构地区：[1]常州市第一人民医院(苏州大学第三附属医院),213000

出　　处：《神经疾病与精神卫生》2005年第4期257-259,共3页Journal of Neuroscience and Mental Health

摘　　要：目的探讨氯化锂-匹罗卡品致癫大鼠脑髓鞘转录因子1基因表达变化及其意义。方法以氯化锂、匹罗卡品对雄性成年SD大鼠先后腹腔注射,制成癫痫持续状态动物模型;采用5′末端标记地高辛的寡核苷酸探针荧光原位核酸分子杂交检测癫性发作后早期大鼠大脑皮质MyT1 mRNA阳性细胞数量。结果与对照组相比,癫痫后1d组大鼠脑皮质MyT1 mRNA阳性细胞数减少(P<0.05);其他各组大鼠脑皮质MyT1 mRNA阳性细胞数都有明显的增加,其中癫痫后7d和14d组MyT1mRNA阳性细胞数都有非常显著的增加(P<0.05,P<0.01)。结论氯化锂-匹罗卡品致癫大鼠早期大脑MyT1 mRNA表达增加,并有时程性变化,提示与早期脑损伤修复有关。Objective To explore the gene expression of myelin transcription factor 1 and its significance in brain of epileptic rats induced by chloride lithium pilocarpine. Methods Established epilepsy continuing status model of male adult SD rats by intraperitoneal injection with chloride lithium and pilocarpine. Then determinated MyTlmRNA-positive cells in cortex of epileptic SD rats by fluorescent in situ hybridization with the dig-labeled oligonucleotides. Results Compared with control group, the number of MyT1 mRNA- positive cells within cortex of epileptic rats of 1 day group decreased significantly at 1 day after epilepsy （P〈 0.05）. The number of MyT1 mRNA-positive cells of other groups has significant increase,among of which the number of MyT1 mRNA -positive cells at 7 and 14 days after epilepsy had increased significantly （P 〈 0.05 ,P 〈 0.01 ）. Conclusions The early phase expression of MyT1 mRNA in brain of chloride lithium pilocarpine induces epileptic rats increase and has alterations at each time-point,and maybe contribute to repairing of early phase brain injury.

关 键 词：癫痫 髓鞘转录因子1 基因表达 氯化锂 匹罗卡品 

分 类 号：R742.1[医药卫生—神经病学与精神病学]