苏木醇提取物对自身免疫性重症肌无力小鼠淋巴细胞功能影响的实验研究  

The effect of ethanol extracts of caesalpinia sappan L. on lymphocyte function in EAMG rats

作　　者：赖成虹[1] 李作孝[1] 江应政 邬于川[3] 

机构地区：[1]四川省泸州医学院附属医院神经内科,646000 [2]四川省安岳县中医医院 [3]四川省泸州医学院免疫教研室

出　　处：《中国现代神经疾病杂志》2005年第5期330-333,共4页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：四川省中医药管理局(2004A36);四川省泸州市科学技术局资助项目

摘　　要：目的采用中药苏木醇提取物治疗实验性自身免疫性重症肌无力(EAMG)小鼠,观察其T淋巴细胞和B淋巴细胞功能的变化情况,探讨苏木治疗重症肌无力的作用机制。方法80只昆明小鼠被随机分为EAMG治疗组(20只)、EAMG对照组(20只)、佐剂对照组(20只)和正常对照组(20只)。采用乙酰胆碱受体(AChR)加完全福氏佐剂(CFA)的方法多次免疫小鼠,复制实验性自身免疫性重症肌无力小鼠模型;佐剂对照组模型制备成功后仅予完全福氏佐剂。所有小鼠均于末次免疫后7～14d行游泳试验和肌电图检查,进行模型鉴定。EAMG治疗组小鼠于末次免疫后第15天起,每天胃内灌注苏木醇提取物0.2ml/只,EAMG对照组、佐剂对照组及正常对照组胃内灌注等量生理盐水,连续给予4周后处死小鼠,应用MTT比色法检测T淋巴细胞和B淋巴细胞转化功能。结果(1)EAMG对照组小鼠的T淋巴细胞吸光度差值为(0.257±0.025),较佐剂对照组(0.147±0.022)及正常对照组(0.154±0.017)明显增高(P<0.01);EAMG治疗组小鼠的吸光度差值为(0.174±0.040),较EAMG对照组明显降低(P<0.01),但仍高于佐剂对照组和正常对照组(P<0.05);佐剂对照组与正常对照组相比,差异无显著性意义(P>0.05)。(2)EAMG对照组小鼠的B淋巴细胞吸光度差值(0.789±0.101)较佐剂对照组(0.502±0.125)及正常对照组(0.493±0.09)明显增高(P<0.01);EAMG治疗组小鼠的吸光度差值为(0.590±0.120),较EAMG对照组明显降低(P<0.01),但仍高于佐剂对照组及正常对照组(P<0.05);佐剂对照组与正常对照组间相比,差异无显著性意义(P>0.05)。结论苏木具有下调实验性自身免疫性重症肌无力小鼠淋巴细胞功能,抑制N2乙酰胆碱受体抗原诱导特异性免疫反应。Objectlve To explore the mechanism ot caesalpmia sappan L. （C5） in the treatment ot myasthenia gravis （MG）, the changing patterns of T lymphocyte and B lymphocyte functions were observed in experimental autoimmune myasthenia gravis （EAMG） rats with ethanol extracts of caesalpinia sappan L. Methods Eighty rats were randomly divide into EAMG therapeutic group （n = 20）, EAMG contol group （n = 20）, CFA adjuvant control group （n = 20） and nomal control group （n ： 20）. The EAMG rats were multi-immu- nized by acetylcholine receptor （AChR） combined with complete Freud＇s adjuvant （CFA）, then the EAMG rat models were duplicated. Only CFA was administrated to the rats in CFA control group. All of the rats were identified by swimming test and electromyogram in 7-14 d after the last immunization. Each of the rats in EAMG therapeutic group was perfused CS extract 0.2 ml/d into stomach and 0.2 ml/d normal saline was per- fused into stomach of each rats in EAMG control, adjuvant control and normal control group since the fifteenth day after the last immunization. The perfusion was lasting 4 weeks, then the rats were sacrificed and the transformation function of T lymphocyte and B lymphocyte was measured by MTr colorimetry. Results 1） Comparing the optic density （OD） value variety of T lymphocyte in each group, its value in EAMG control group （0.257±0.025） was much higher than that in CFA control group （0.147±0.022） and normal control group （0.154±0.017） （P〈 0.01）; the OD in EAMG therapeutic group （0.174±0.040） was much lower than that in EAMG control group （P〈 0.01）, but still higher than that in CFA control group and normal control group （P 〈 0.05）; no significant difference was found in between the CFA control group and normal control group （P〉 0.05）. 2） Comparing the OD value variety of B lymphocyte in each group, the OD value in EAMG control group （0.789±0.101） was much higher than that in CFA control group （0.502±0.125） and

关 键 词：苏木 重症肌无力 T淋巴细胞 B淋巴细胞 疾病模型 动物 实验性自身免疫性重症肌无力 B淋巴细胞功能 苏木醇提取物 昆明小鼠 功能影响 正常对照组 实验研 淋巴细胞转化功能 乙酰胆碱受体 

分 类 号：R746.1[医药卫生—神经病学与精神病学] R329.24[医药卫生—临床医学]