人乳腺癌细胞凋亡与去磷酸化Rb蛋白和PCNA的关系  

The relationship between cell apoptosis and dephosphorylated Rb protein and PCNA in human breast cancer

作　　者：王文武[1] 欧阳学农[1] 姜浩[2] 

机构地区：[1]国家中西医结合肿瘤重点专科南京军区福州总医院肿瘤科,福建福州350025 [2]南华大学附属第一医院,湖南衡阳421001

出　　处：《中国癌症杂志》2005年第5期429-431,共3页China Oncology

基　　金：湖南省卫生厅课题(2001-Y58)

摘　　要：目的:研究乳腺癌细胞凋亡与去磷酸化Rb蛋白和增殖细胞核抗原(PCNA)的关系。方法:应用MTT比色法检测多柔比星(又名阿霉素,ADR)对体外培养的MCF-7/S细胞增殖抑制作用,应用流式细胞术检测ADR诱导乳腺癌细胞凋亡,采用免疫组织化学法检测ADR作用前后去磷酸化Rb蛋白和PCNA的表达。结果:ADR抑制MCF-7/S细胞增殖,呈剂量依赖性;ADR作用组MCF-7/S细胞的凋亡率及去磷酸化Rb蛋白的表达量与对照组相比明显增高(P<0.01);PCNA阳性表达率与对照组相比明显降低(P<0.01)。结论:ADR作用组,MCF-7/S细胞的凋亡率(AR)与去磷酸化Rb蛋白的表达量呈正相关,与增殖细胞核抗原的阳性表达率呈负相关。Purpose：To investigate the relationship between cell apoptosis and dephosphorylated Rb protein and Proliferating cell muclear antigen（PCNA）in human breast cancer.Methods：MTT colorimetric assay was applied to examine the growth inhibition,and the apoptosis was determined by flow cytometry（FCM）：the expressive levels of dephosphorylated Rb protein and PCNA were detected with immunocytochemistry.Results：MTT assay revealed that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration（IC50）value was 0.128mg.L^-1.Tumor cell apototic rate（AR） in ADR group（=0.259） was significantly higher than that in control group（=0.045）（P〈0.01）.The expressive levels of dephosphorylated Rb protein in ADRgroup （MOD×area=986.8±207.4）was significantly higher than that in control group（MOD×area=131.7±31.9）（P〈0.01）,PCNA positive expression rate in ADR GROUP（=0.3371）was significantly lower than that in control group（=0.5152）（P〈0.01）.Conclusions：In ADR group,there was significant positive correlation between AR and the expressive levels of dephosphorylated Rb protein,but there was significant negative correlation between AR and PCNA.

关 键 词：去磷酸化RB蛋白 乳腺肿瘤 凋亡 阿霉素 增殖细胞核抗原 

分 类 号：R73-361[医药卫生—肿瘤]