电化学治疗昆明小鼠肉瘤及其机理分析  

Electrochemotherapy and Its Mechanism for Sarcoma of KM Mice

作　　者：杨孔[1] 何苗[1] 王子淑[1] 邹方东[1] 岳碧松[1] 

机构地区：[1]四川大学生命科学学院

出　　处：《生物医学工程学杂志》2005年第5期914-917,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(60071024)

摘　　要：研究了电化学治疗昆明小鼠肉瘤的疗效,并分析其机理。在体外将S-180细胞用不同参数的电场处理,研究适合电化学治疗的电场条件。通过复制肉瘤模型,将肉瘤小鼠随机分成4组:对照组、电疗组、化疗组、电化疗组。研究了不同处理组的肿瘤抑瘤率、治愈率以及小鼠的自由基代谢水平。结果电化疗组的抑瘤率、治愈率都显著高于化疗组和电疗组(P<0.05),电化疗组小鼠受到氧自由基的攻击显著降低,免疫力提高。分析机理发现,电化学治疗肿瘤的机理可能至少涉及细胞膜通透性提高、细胞的耐药性降低、机体的免疫力提高三个方面。In this paper are analyzed the effect of electrochemotherapy and its mechanism. The favorable parameter of electric pulses（EP） was studied in vitro using the S-180 cells exposed to various EP. After the tumor model was copied, the tumor-bearing mice were randomly divided into four groups., control, chemotherapy, electrotherapy, and electrochemotherapy. The tumor inhibitory ratio, the cure ratio and the level of oxygen free radicals （OFR） were determined. The inhibitory ratio and cure ratio of electrochemotherapy group were significantly higher than those in the chemotherapy, electrotherapy and control groups（P〈0.05）. The injury of OFR was decreased while the immunological competence was increased. The mechanism of electrochemotherapy may be related with the enhancement of cell membrane permeability, the depression of drug resistance, the improvement of immunological competence, and so on.

关 键 词：电化学治疗 肉瘤 疗效 机理 电化学治疗 小鼠肉瘤 机理 昆明 S-180细胞 细胞膜通透性 氧自由基 电化疗 代谢水平 

分 类 号：R73-3[医药卫生—肿瘤]