局灶性脑缺血后室管膜 /室下区细胞迁移到梗塞区周围并分化为神经元和星形胶质细胞(英文)  

Ependymal/subventricular zone cells migrate to the peri-infarct region and differentiate into neurons and astrocytes after focal cerebral ischemia in adult rats

作　　者：张蓬勃[1,2] 刘勇[1] 李捷[1] 康前雁[1] 田英芳[1] 陈新林[1] 赵建军[1] 石秦东[1] 宋土生[1] 钱亦华[1] 

机构地区：[1]西安交通大学医学院环境与疾病相关基因教育部重点实验室,神经科学研究中心 [2]西安交通大学第二医院麻醉科,陕西西安710004

出　　处：《第一军医大学学报》2005年第10期1201-1206,共6页Journal of First Military Medical University

基　　金：This work was supported by Grant from National Natural ScienceFoundation of China (30200291, 30170300) and Natural Science Foundation of Shaanxi Province,China(2002C250,2001SM63).

摘　　要：目的研究局灶性脑缺血后室管膜/室下区细胞的迁移分化,揭示梗塞区周围新生细胞的来源.方法大脑中动脉阻塞前,将10μl 0.2%的荧光染料DiI注射于体质量250～350 g的雄性SD大鼠侧脑室以预标记室管膜/室下区细胞.脑缺血后,采用累积式的BrdU标记方法标记新生细胞并通过双重免疫荧光染色确定细胞分化.标记的细胞通过激光共聚焦显微镜观察.结果在非缺血对照大鼠,DiI标记细胞定居于室管膜/室下区.局灶性脑缺血后,DiI标记细胞出现于胼胝体,邻近的纹状体和皮质.此外,缺血14 d后,梗塞区周围纹状体和皮质内可见一些DiI/BrdU/GFAP或DiI/BrdU/NeuN三重标记阳性细胞.结论局灶性脑缺血后,室管膜/室下区细胞迁移到梗塞区周围并分化成神经元和星形胶质细胞,这一发现对于理解成体神经干细胞的起源和开发促进脑损伤后内源性神经发生的新措施具有重要意义.Objective To investigate the migration and differentiation of ependymal/subventricular zone cells after focal cerebral ischemia in rats, and reveal the origin of the newly generated neural cells in the peri-infarct region. Methods Normal adult male Sprague Dawley rats weighing 250-350 g were used in this study. Before middle cerebral artery occlusion （MCAO）, 10 μl of 0.2% DiI was injected into the lateral ventricle for prelabeling the ependymal/subventricular zone cells.After ischemia, cumulative BrdU labeling was employed to detect the newly generated cells and double immunofluorescent staining to identify cell differentiation. The labeled cells were observed with laser confocal microscopy. Results In the non-ischemic control rats, DiI-labeled cells resided in the ependyma/subventricular zone. After focal cerebral ischemia,DiI-labeled cells were found in the corpus callosum, adjacent striatum and cortex, and some DiI/BrdU/glial fibrillary acidic protein （GFAP）-positive cells or DiI/BrdU/neuronal nuclear antigen （NeuN）-positive cells were observed in the peri-infarct region in the striatum or cortex since day 14 after MCAO. Conclusion After focal cerebral ischemia,ependymal/subventricular zone cells migrate into the peri-infarct region where they differentiate into neurons and astrocytes.This finding may be important for understanding the source of adult neural stem cells and for developing new therapeutic intervention strategy through enhancing endogenous neurogenesis after brain injury.

关 键 词：神经干细胞 室管膜细胞 室下区细胞 神经发生 局灶性脑缺血 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]