山羊基因组文库的构建研究  

Construction of Goat Genomic Library

作　　者：唐爽[1] 安志兴[1] 张明烽[1] 宋红卫[1] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨陵712100

出　　处：《中国农学通报》2005年第10期19-20,65,共3页Chinese Agricultural Science Bulletin

基　　金：国家奶业重大科技专项(2002BA518A01-2)。

摘　　要：构建了山羊基因组文库,为筛选山羊乳蛋白基因,构建山羊乳蛋白基因定点整合的转基因敲入载体奠定基础.全血提取基因组DNA,Sau3AI部分酶切,分级分离回收15～23 kb片段,将片段插入Lambda BlueSTAR BamHI vector,连接并包装后,NotI酶切鉴定.结果表明,文库滴度为3.4×106;共得到1.7×106个有效克隆,插入片断长度在15～23kb范围,成功构建了西农萨能奶山羊基因组文库.Goat genomic DNA library was constructed for screening milk-protein-encoding gene of goat and construction of knock-in vector. Genomic DNA was prepared from blood sample, partially digested with Sau3AI.15-23kb fragment was fractionated and ligated to Lambda BlueSTAR BamHI Arms. Titer of the library is 3.4×10^6; 1.7×10^6 clones were obtained with inserts size ranging from 15-23kb.

关 键 词：基因组文库 西农萨能奶山羊 萨能奶山羊 基因组文库 构建 乳蛋白基因 基因组DNA 基因定点整合 LAMBDA VECTOR 酶切鉴定 

分 类 号：S827.32[农业科学—畜牧学] Q785[农业科学—畜牧兽医]