黑斑蛙3个Sox基因HMG-box区的序列分析  

PCR-SSCP and sequence analysis of Sox gene in the Rana Nigromaculata

作　　者：刘先英[1] 季代丽[1] 李洁[1] 聂刘旺[1] 

机构地区：[1]安徽师范大学生命科学学院,芜湖241000

出　　处：《生物学杂志》2005年第5期27-30,共4页Journal of Biology

基　　金：安徽省教育厅自然科学基金重点项目(2002kj128);安徽省自然科学基金(NO.01043202)

摘　　要：参照人SRY基因HMG-box保守区的序列,设计一对兼并引物,扩增了黑斑蛙Sox基因并对扩增产物进行了克隆及测序。结果在雌雄个体中共筛选出三个不同的Sox基因,rnSox3,rnSox21,rnSox4其序列与人相应SOX基因的相似性分别为86%、82%、83%,与人类相应SOX基因编码氨基酸的一致性分别为94%、93%、78%。本研究为探索黑斑蛙的性别决定机制提供了分子资料。The Sox gene of Rana Nigromaculata with degenerate primers which specially amplify the conservation sequence of human HMG- box gene was amplified. Three different Sox genes were obtained from male and female body. The identify of possible sequence of obtained SOX to that of human is 82 %, 83 %, 86 %, respectively. The identify of possible amino acid sequence of obtained SOX to that of human is 94 %, 93 %, 78 %, respectively. The data for the study on Sex - determining mechanism of Rana Nigromaculata were supplied.

关 键 词：SOX基因 黑斑蛙 简并PCR 

分 类 号：Q755[生物学—分子生物学]