Manumycin通过诱导细胞凋亡抑制人胰腺导管癌细胞Panc-1活性  

Manumycin inhibits activity of pancreatic duct cancer cell line Panc-1 via inducing apoptosis

作　　者：杨惠玲[1] 陶祥[1] 杨林[2] 郑芹[1] 

机构地区：[1]中山大学病理生理学教研室,广东广州510080 [2]中山大学附属第三医院传染病科,广东广州510630

出　　处：《中国病理生理杂志》2005年第10期1888-1891,共4页Chinese Journal of Pathophysiology

基　　金：广东省重大科技专项基金资助项目(No.2004A30801006);国际合作项目(2004B50301003)

摘　　要：目的:观察manumycin对人胰腺导管癌细胞Panc-1的抑制效应,并探讨其诱导细胞凋亡是否经 p38MAPK介导。方法:用MTT法检测manumycin对Panc-1细胞的抑癌作用。用caspase-3活性检测试剂盒定量检测 manumycin诱导细胞凋亡的水平及评估特异性的p38MAPK抑制剂SB203580对它的影响。结果:经manumycin (6／μmol／L、18／μmol／L、54 μmol／L)处理Panc-1细胞24 h,对Panc-1细胞生长具有明显的抑制作用,其抑制率分别为 8．9％、21．9％和67．0％,其中后二者的细胞活性与对照组相比有显著差异(P<0．01),呈量效关系。用药24 h的IC50 为34．7μmol／L。同时,此药物可明显增加caspase-3的活性,且这一效应可部分地被p38抑制剂SB203580阻断。结 论:Manumycin可通过诱导Panc-1细胞凋亡而产生抑癌作用,p38MAPK是manumycin诱导细胞凋亡的通路之一。AIM： In this study, we investigated the anticancer effect and mechanisms of manumycin on pancreatic cancer cell line -Panc - 1 and the role of p38MAPK pathway in apoptosis. METHODS： The test of anticancer effect was performed by MTT assay. Apoptosis was induced in the cells by manumycin and then treated with SB203580, a specific p38MAPK inhibitor. A quantitative caspase - 3 activity assay kit was used in this experiment. RESULTS： Manumyein （6μmol/L, 18μmol/L, 54μmol/ L） significantly inhibited cell growth of pancreatic cancer cell line Panc - 1. The inhibition rates 24 h after treatment with 6 μmol/ L, 18 μmol/L and 54 μmol/L manumycin were 8.9%, 21.9% and 67.0%, respectively. Compared with the control group, the survival levels of the last two groups were of significant statistical difference （ P 〈 0.01 ）. The anticancer effects also showed dosage - effect relationship, the v.,due of IC50 24 h after treatment was 34.7 μmol/L. In addition, this reagent simultaneously activated caspase - 3 protein, which was partly blocked by p38MAPK specific inhibitor, SB203580. CONCLUSION： Manumycin exerted anticancer effect on Pane - 1 cell line via inducing cell apoptosis, which was partly regulated by p38MAPK.

关 键 词：MANUMYCIN 胰腺导管癌 细胞凋亡 P38MAP激酶 

分 类 号：R735.905.3[医药卫生—肿瘤]