外源性Rb基因对U937细胞泡沫化过程中载脂蛋白B的影响  

Effects of exogenous Rb gene on apoB in U937 cells during formation of foam cells

作　　者：闾宏伟[1] 袁洪[1] 阳国平[1] 陈淑华[2] 杨向东[3] 

机构地区：[1]中南大学湘雅三医院医学实验中心,湖南长沙410013 [2]中南大学 [3]南华大学心血管病研究所,湖南衡阳421001

出　　处：《中国病理生理杂志》2005年第10期1909-1913,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助课题(No.30200103);湖南省教育厅青年基金资助项目(No.02B039)

摘　　要：目的:研究Rb基因在U937细胞泡沫化过程中对细胞内载脂蛋白B含量的影响,初步探讨Rb基因 在泡沫细胞形成过程中的作用。方法:采用氧化型低密度脂蛋白(Ox-LDL)孵育U937细胞,以及Ox-LDL孵育同时 导入外源性Rb基因转染U937细胞,分别于处理0 h、12 h、24 h收集细胞,半定量RT-PER与流式细胞术检测Rb mRNA和蛋白的表达,流式细胞术检测细胞内载脂蛋白B的含量。平行实验重复3次,进行统计学方差分析。结果:氧 化型低密度脂蛋白孵育U937细胞泡沫化过程中,12 h、24 h Rb基因的表达显著低于对照组0 h(P<0．05);细胞内载 脂蛋白B含量则显著高于对照组0 h(P<0．05)。而U937细胞在导人外源性Rh基因后,12 h、24 h细胞内的Rb基因 的表达显著高于对照组0 h(P<0．05);细胞内载脂蛋白B含量则显著低于对照组0 h(P<0．05)。结论:外源性Rb 基因的导入可以下调细胞内载脂蛋白B的含量,表明Rb基因可能与单核细胞源性泡沫细胞形成有关,可能是影响 泡沫细胞形成的相关基因的敏感候选基因之一。AIM： To investigate the effects of exogenous retinoblastoma （Rb） gene on apolipoprotein B （apoB） during the transformation of U937 cells into foam cells and to explore the function of Rb gene in this process. METHODS： In one group, human myeloid leukemia U937 cells were incubated with 80 mg/L oxidative modifications of low density lipoprotein （Ox - LDL） for 0, 12 and 24 hours, respectively. In the other group, the U937 cells were treated similarly, except that the cells were also trans- fected with exogenous Rb gene via a recombinant adenovirus vector. The expression levels of Rb gene and the contents of apoB in both groups were detected by RTPCR and flow cytometry. The same experiments were repeated three times. RESULTS： The expression levels of Rb in U937 cells within 12 h and 24 h incubations with Ox - LDL were significantly lower than that at 0 h （ P 〈 0.05）, and the contents of apoB within 12 h and 24 h incubation with Ox - LDL were significantly higher than that at 0 h （ P 〈 0.05）. The U937 cells transfected with exogenous Rb gene via a recombinant adenovirus vector, however, showed that the expression levels of Rb gene within 12 h and 24 h incubation with Ox - LDL were significantly higher than that at 0 h （ P 〈 0.05）, and the content of apoB within 12 h and 24 h incubation with Ox-LDL were significantly lower than that at 0 h （P 〈 0.05）. CONCLUSlONS： The introduction of exogenous Rb gene into human myeloid leukemia U937 cells down-regulates the content of apoB, suggesting that the Rb gene may play certain roles in the transformation of monocytes into foam cells and may be considered as one of the sensitive candidate genes affecting the formation of foam cells.

关 键 词：U937细胞 基因 视网膜母细胞瘤 载脂蛋白B类 

分 类 号：R363[医药卫生—病理学]