多胺对大鼠缺氧-复氧心肌细胞内钙的影响  

Effect of polyamines on intracellular calcium of the rat cardiomyocytes with anoxia and reoxygenation

作　　者：赵雅君[1] 徐长庆[1] 时飒[1] 孙宏力[2] 王宁[2] 李宝馨[2] 王玲[2] 杨宝峰[2] RuiWang 

机构地区：[1]哈尔滨医科大学病理生理教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学药理教研室,黑龙江哈尔滨150086 [3]加拿大Saskatchewan大学生理系

出　　处：《中国病理生理杂志》2005年第10期1938-1941,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370577);黑龙江省教育厅海外学人科研(合作)基金资助基金项目(No.1053HQ010)

摘　　要：目的:观察外源性低浓度多胺对大鼠缺氧-复氧心肌细胞钙超载的影响。方法:酶解分离大鼠心室 肌细胞,用正常氧合Tyrode液灌流8 min,换为缺氧液灌流32 min,再转换为正常氧合Tyrode液灌流8 min,复制心肌 缺氧-复氧模型。分别在缺氧前给予精胺,缺氧-复氧后给予精胺、精脒、腐胺。应用激光扫描共聚焦显微镜 (LSCM)连续观察细胞内钙荧光强度的动态变化。结果:精胺(1 mol/L)对正常静息状态下大鼠心室肌[Ca2+]i无影 响。缺氧前给予精胺,可取消复氧引起的心肌[Ca2+]i增高;缺氧-复氧后给予精胺、精脒、腐胺对缺氧-复氧引起 [Ca2+]i升高也有不同程度的降低作用,其中以精胺的作用最强。复氧后给予精胺降低缺氧-复氧[Ca2+]i升高的 作用小于缺氧前给予。结论:缺氧前给予精胺可拮抗缺氧-复氧心肌细胞钙超载发生;复氧后给予精胺可使缺氧- 复氧心肌细胞钙超载减轻,但其作用力度不如缺氧前给药。多胺拮抗缺氧-复氧心肌细胞钙超载的作用以精胺> 精脒>腐胺的顺序递减。AIM： To study the effect of extrogenous low concentration polyamine on cardiomyecyte calcium overload caused by anoxia and reoxygenation. METHODS： Enzymatically isolated rat ventricular myocytes were perfused with normal Tyrode solution for 8 min, then change to anoxia solution for 32 min, at last back to normal Tyrode solution perfusion for 8 min to establish the cardiomyocyte model of anoxia and reoxygenation. Spermine was added extracellularly to the bath before anoxia and spermine, spermidine or putrescine was added extracellularly after reoxygenation. Intracellular calcium fluorescence intensity （FI） was measured continuously by laser scan confocal microscope （LSCM）. RESULTS： In the unstimulated state, exogenous spermine （1 mol/ L） did not change resting [Ca^2＋]i in the rat cardiomyocytes. Adding spermine before anoxia antagonized the [Ca^2＋]i elevating caused by anoxia/reoxygenation. Adding spermine after reoxygenation also lowed the enhanced [ Ca^2＋]i caused by reoxygenation. Considering the potency of two conditions, the former was more efficacious than the later. Spermidine and putrescine also lowed the enhanced [Ca^2＋]i caused by reoxygenation, but they were less efficacious than spormine. CONCLUSIONS： The results indicate that spormine given before anoxia or after reoxygenation, antagonized or lowed the cardiomyecytes calcium overload caused by anoxia/reoxygenation, but the later was weaker than the former. The order of potency of the polyamine lighten cardiomyecytes calcium overload caused by anoxia/reoxygenation was spermine 〉 spermindine 〉 putrescine.

关 键 词：聚胺类 心肌 缺氧 钙 

分 类 号：R363[医药卫生—病理学]