镍冶炼烟尘致NIH/3T3细胞DNA损伤的实验研究  

Experimental study on the DNA damage of NIH/3T3 cells induced by nickel-refining dusts

作　　者：吴永会[1] 王军[1] 赵光[1] 于翠平[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,150086

出　　处：《中华劳动卫生职业病杂志》2005年第5期326-328,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目(30371205)

摘　　要：目的研究镍冶炼烟尘致DNA损伤作用。方法采用单细胞凝胶电泳(SCGE)技术,检测2份镍冶炼烟尘对小鼠NIH/3T3细胞的DNA损伤。结果相同染毒时间内,随着染毒剂量(除样品2中50.0μg/ml剂量染毒24h组)加大,细胞拖尾率增高,DNA断裂程度在4h达到高峰,分别为35.5%、69.7%、85.2%、41.3%、75.7%、89.2%。各受试物在相同剂量下,染毒4h时均较染毒2h和染毒24h细胞拖尾率高。NIH/3T3细胞受到镍冶炼烟尘的受试物作用后,在染毒2h,样品1、样品2各剂量组的细胞彗星尾DNA含量、尾长(除12.5μg/ml剂量组外)、尾动差(除样品1的12.5μg/ml剂量组外)均明显高于阴性对照组,差异有统计学意义(P<0.05)。在染毒4h,两样品对细胞DNA的损伤达到高峰,各剂量组的3个指标与对照组的差异有统计学意义(P<0.05)。染毒时间延长至24h,上述3个指标改变程度均较染毒4h减弱,样品1的彗尾长与对照组的差异无统计学意义(P>0.05)。结论镍冶炼烟尘可不同程度致NIH/3T3细胞DNA损伤。Objective To study the effects of nickel-refining dusts on DNA damage in mouse NIH/3T3 cell. Method DNA damage of mouse NIH/3T3 cell induced by two nickel-refining dust samples was determined with single cell gel electrophoresis （SCGE） technique. Results （ 1 ） Under the condition of the same treatment time,the tailed cell（ % ） of NIH/3T3 cells increased with the increase in doses of nickel-refining dusts（35.5% , 69.7% ,85.2% ,41.3% ,75.7% ,89.2% respectively except for sample 2.50μg/ml, 24 h group）,and DNA strand breaks reached the peak value at 4 h of exposure; （2）When we treated the NIH/3T3 cells with the same dose of nickel-refining dusts,the tail rate at 4 h was higher than those at 2 h and 24 h of exposure; （3）Both sample 1 and sample 2 with different doses of nickel-refining dusts could induce higher comet tail,DNA% ,tail length（except for 12.5μg/ml）, extent of TM（ except for sample 1,12.5 μg/ml） than in control group（ P 〈 0.05）. The DNA damage range was significantly increased in different tested doses of nickel-refining dusts and the damage range reached the peak value when the cells were treated for 4 h. Conclusion Nickel-refining dusts could induce different degree DNA damage in NIH/3T3 cells.

关 键 词：烟尘 镍冶炼 DNA损伤 彗星试验 NIH/3T3细胞 细胞DNA损伤 镍冶炼烟尘 单细胞凝胶电泳(SCGE) 实验研 染毒时间 DNA损伤作用 染毒剂量 DNA断裂 

分 类 号：R734.2[医药卫生—肿瘤]