硒与肿瘤 Ⅲ、植瘤小鼠给抑瘤剂量硒后胞浆中^(75)Se的分布与含硒蛋白变化  

SELENIUM AND CANCER Ⅲ. THE DISTRIBUTION OF 75-SE, GPX AND SELENOPROTEINS IN THE CYTOSQLS OF TUMOR BEARING NICE TREATED WITH ANTICARCINOGENIC SELENIUM

作　　者：颜学先[1] 王鼎年[1] 

机构地区：[1]重庆医科大学基础核医学教研室

出　　处：《重庆医科大学学报》1989年第2期85-89,共5页Journal of Chongqing Medical University

基　　金：四川省卫生厅科研课题

摘　　要：植H615肝癌实体瘤的615纯种小鼠,于植瘤后第15天开始每隔5天腹腔注射2ugSe/g体重的亚硒酸钠及示踪剂量^(75)Se,给硒6次后处死,通过葡聚糖凝胶层析观察^(75)Se在红细胞、肝及瘤细胞胞浆中的分布。结果发现:胞浆中不仅GPX上结合有硒,也存在着其它的含硒蛋白质;营养水平的硒和抑瘤水平的硒在红细胞、肝及瘤胞浆中的分布情况不同;同一水平的硒在植瘤和未植瘤鼠上述胞浆内的分布也不同。并伴有GPX和其它含硒蛋白质改变。2 ppm selenium as Na2SeO3 and tracer 75-Se-selenite was administered to 615 mire inoculated with liver solid tumor six times over a 40-day period by ip. iniection. All mice were killed on the 45th day following tumor cell inoculation. The cytosols of RBC, liver and tumor cell were chromatographed on a Sephadex G-150 column separately. The 75-Se cpm,GPX activity and absorbance at 280 (or 540) nm of each collection were determined. The results showed that several Se-containing proteins except GPX exist in the cytosols; There are some differences about distribution of 75-Se in all of the three oytosols between the mice given nutritional or aaticarcinogenic selenium and with or without bearing tumor; The GPX and selenoproteins have also some; significant chauges. It is suggested chat these beh a-viors are probably responsible for this trace element's anticarcinogenic properties

关 键 词：硒 肝肿瘤 含硒蛋白 抑瘤 

分 类 号：R735.705[医药卫生—肿瘤]