高效液相色谱法测定动物性食品中四环素类药物的残留  

Determination of Tetracyclines Residues in Animal Food by RP-HPLC

作　　者：韦田[1] 田淑琴[1] 李远森[1] 龙江[1] 曹雨辰[1] 刑莉莉[1] 王庆福[1] 宋慧[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《现代畜牧兽医》2005年第10期42-44,共3页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：采用高效液相色谱法进行四环素类抗生素药物残留量的测定。样品经提取,微孔滤膜过滤,超声波排气,反相色谱分离。色谱柱为AgilentExtend-C18(150×4.6nm,5umi.d)柱,以乙腈-磷酸二氢钠(体积比69∶31)为流动相,紫外检测器检测波长为355nm,以0.8ml/min的流速,采用内加法[1]测定了25个鸡肝样品中土霉素、四环素、金霉素的残留量。测定结果土霉素、四环素、金霉素残留平均值分别为40.7、28.04、71.5μg/kg(n=25)。实验结果表明:16%的样品中有残留,其中土霉素超标占残留样中的25%、金霉素超标占残留样中的50%,四环素未测出超标。A method for the determination of tetracyclines residues in animal food by RP-HPLC.The samples were separated with reversed phase chromatography after soaked, chromatography, TCs were successfully separate on Agilent Extend-C18 column（150×4.6nm, 5um i.d）.using acetoitrile-NaH2PO4.2H2O （31：69 volume ratio） as mobile phase at a flow rate of 0.8ml/min and detected at 355nm. Inthis experiment 25 pieces of livers of poultry were measured with the standard added method. The results were as follows; The percentage of OTC was 40.7μg/kg, the percentage of TC was 20.08μg/kg and the percentage of CTC was 71.5μg/kg（n=25）, It can be seen from the results ,there was antibiotic in 16% of the samples, of OTC was higher than the lawed-level. and 50% of the samples whose percentage of CTC was higher than the lawed -level, but,all samples whose percentage of TC wash＇t higher than the lawed-level.

关 键 词：兽药残留 四环素族抗生素 高效液相色谱法 

分 类 号：S859.796[农业科学—临床兽医学]