难治性癫痫患者致痫灶细胞凋亡现象的研究  

Study on apoptosis in epileptic focus of patients with intractable epilepsy

作　　者：高旭[1] 张华[1] 井晓荣[1] 田爽[2] 高国栋[1] 

机构地区：[1]第四军医大学唐都医院神经外科,全军微创神经外科中心,功能性脑疾病研究所,西安710038 [2]西京医院妇产科

出　　处：《立体定向和功能性神经外科杂志》2005年第5期260-263,共4页Chinese Journal of Stereotactic and Functional Neurosurgery

摘　　要：目的观察难治性癫痫患者手术切除癫痫灶中细胞凋亡现象,探讨神经元凋亡在癫痫发病机制中的意义,为临床治疗提供新的理论依据。方法收集16例经手术治疗的难治性癫痫患者致痫灶标本,在对临床资料全面分析的基础上,应用光镜、电镜及凋亡细胞DNA原位末端标记法(TUNEL)观察标本中存在的细胞凋亡现象。结果致痫灶周围神经元普遍固缩、减少,反应性胶质细胞增生。TUNEL阳性细胞数量较对照组显著增高(P<0.01),且绝大部分为神经元。结论难治性癫痫患者致痫灶周围存在细胞凋亡现象,神经元凋亡可能参与癫痫的发病过程。Objective To observe apoptosis in surgically resected epileptic focus of patients with intractable epilepsy and explore its roles in epileptic pathogenesis,in order to provide a fresh theory for clinical therapy. Methods The specimens from 16 patients with intractable epilepsy were collected. With light electron microscope and TUNEL method,apoptosis were observed. Results The loss and pyknosis of neurons and the presence of reactive astrocytes seemed to be a common pathological characteristics. There was a significant difference （P〈 0.01） in the number of TUNEL positive cells, most of which were neurons, between the patients with intractable epilepsy and the controls. Conclusion Apoptotic neurons appeared around epileptic focus of patients with intractable epilepsy, and apoptosis may relate to the pathogenesis of epilepsy.

关 键 词：难治性癫痫 致痫灶 凋亡 

分 类 号：R742.1[医药卫生—神经病学与精神病学]