谷氨酸转运体在全脑缺血性癫痫中作用的研究  

The role of glutamate transporters in Epileptic rats induced by chest compression global cerebral ischemia

作　　者：邱永明[1] 陆兆丰[1] 江基尧[1] 罗其中[1] 

机构地区：[1]上海第二医科大学附属仁济医院神经外科,200127

出　　处：《立体定向和功能性神经外科杂志》2005年第5期275-279,共5页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：上海市教委资助项目(编号:04BB27);国家自然科学基金资助项目(编号:30471771)

摘　　要：目的比较三种谷氨酸转运体在全脑缺血性癫痫中的动态变化特征,为癫痫治疗提供有意义靶点。方法SD大鼠以胸部压迫8分钟造成全脑缺血性癫痫模型,分对照组、假手术组、全脑缺血无癫痫组和全脑缺血癫痫组。后两组又根据脑缺血后时间分为6h,24h,48h,72h,5d,7d组。应用免疫组化法研究谷氨酸转运体EAAT-1,EAAT-2,EAAT-3在海马CA1及皮质区表达;研究病理形态变化,同时测定大鼠脑电图改变。结果大鼠癫痫发生率为64%,全脑缺血癫痫大鼠神经损害较无癫痫组严重。与全脑缺血无癫痫大鼠比较,癫痫大鼠海马CA1及皮质区EAAT-2显著持续降低及EAAT-3表达明显升高。结论大鼠癫痫发生与脑缺血严重程度密切相关。海马CA1及皮层区EAAT-2、EAAT-3表达变化是抗癫痫治疗的作用靶点。Objective The effects of three subtypes of glutamate transporters （EAAT- 1, EAAT- 2, EAAT- 3） on seizure rats induced by global cerebral ischemia were studied comparatively. The targets of this research were to provide another choice for management of cerebral ischemia related seizure. Methods The rat model of global cerebral ischemic seizure was established reliable. The groups included control group; sham group; global cerebral ischemia without seizure （PI）and global cerebral ischemia with seizure（El）. The subgroups were divided into 6h, 24h, 48h, 72h, 5d, 7d, of the later two groups according to the time points after chest compression on rats. The tissue chip was made, the expressions of glutamate transporter EAAT- 1, EAAT- 2, EAAT- 3 in hippocampus CA1 and motor area of frontal cerebral cortex were studied by use of immunohistochemistry. The morphology changes of light and electric microscope were analyzed. The EEG shows were studied. Results The seizure rate was 64 % in this experiment. Compared with PI group, the expression of EAAT- 2 in El group at hippocampus CA1 and cortex region was down - regulation and EAAT - 3 was up - regulation. Conclusion The occurrence of seizure has related to the severity of cerebral ischemia. The changes of EAAT - 2 and EAAT - 3 were the potential point for the treatment of cerebral ischemiarelated seizure.

关 键 词：谷氨酸转运体 全脑缺血 癫痫 病理 

分 类 号：R742.1[医药卫生—神经病学与精神病学]