酪丝亮肽对人肝癌BEL-7402裸鼠移植瘤的抑制作用  

Inhibitory effect of tyroserleutide on nude mice transplanted tumor of hepatic carcinoma human BEL-7402

作　　者：王莉[1] 陆融[1] 王犁明[1] 赵琳[1] 傅正[1] 王松[1] 李会强[1] 杨海贤[2] 章明放[3] 金孟珏[4] 姚智[1] 

机构地区：[1]天津医科大学免疫学教研室,天津300070 [2]天津医科大学电镜中心,天津300070 [3]天津医科大学病理教研室,天津300070 [4]天津医科大学动物中心,天津300070

出　　处：《中国新药与临床杂志》2005年第11期857-861,共5页Chinese Journal of New Drugs and Clinical Remedies

基　　金：国家"863"高技术研究发展计划资助项目(2004AA2Z3170;2005AA2Z3D40);国家教育部重点项目(03007);国家重点基础研究发展规划(973)前期专项(2003CCA04300)

摘　　要：目的:观察酪丝亮肽对人肝癌BEL-7402的抑制作用,并分析其可能机制。方法:制备裸鼠移植瘤模型,随机分为生理盐水、氨基酸混合物、环磷酰胺、酪丝亮肽高低剂量等5组,每组12只,观察酪丝亮肽对人肝癌BEL-7402肿瘤组织坏死和凋亡的影响;电镜观察其对肿瘤细胞超微结构改变;流式细胞技术分析其对细胞周期及细胞凋亡率的影响。结果:酪丝亮肽能够诱导肿瘤细胞坏死和凋亡、细胞周期阻抑、显著抑制肿瘤的生长,给药剂量为80μg.kg-1.d-1和160μg.kg-1.d-1时,肿瘤抑制率分别为42%与58%。结论:酪丝亮肽可以显著抑制人肝癌BEL-7402的生长,作用机制与促进肿瘤细胞的凋亡和坏死有关。AIM ： To investigate the antitumor effect of tyroserleutide on the growth of the human BEL-7402 hepatocarcinoma inoculated into nude mice and explore preliminary mechanism. METHODS：The nude mice bearing xenografts of the human BEL-7402 hepatic carcinoma were given daily ip injections of tyroserleutide or saline or mixture of amino as controls after tumor implantation, altogether about five groups with twelve mice for each. Apoptosis and necrosis were determined with fluorescence-activated cell sorter （FACS） and electron microscopy for detecting cellular microstructural charges. could significantly induce growth in nude mice and RESULTS ： Tyroserleutide the inhibition tumor of changed the ultrastructure and cell cycle of tumor cell. At dose of 80 μg·kg^-1·d^-1and 160 μg·kg^-1·d^-1 it could significantly inhibit tumor growth in nude mice by inhibition rates of 42 % and 58 % respectively. CONCLUSION ： Tyroserleutide can inhibit significantly tumor growth in nude mice. The growth inhibition of the xenografts may concern with the apoptosis of tumor cells induced by tyroserleutide.

关 键 词：癌 肝细胞 细胞凋亡 小鼠 裸 酪丝亮肽 

分 类 号：R73-3[医药卫生—肿瘤] R979.1[医药卫生—临床医学]