高强度聚焦超声治疗对乳腺癌细胞表达免疫抑制因子的影响  

The Effects of High Intensity Focused Ultrasound Treatment on Immunosuppressive Factors of breast cancer

作　　者：周强[1] 伍烽[2] 朱学强[3] 张军[4] 李莉[2] 王智彪[2] 

机构地区：[1]遂宁市人民医院肿瘤科,四川省遂宁市629000 [2]重庆医科大学附属第二医院肿瘤中心医学超声工程研究所 [3]四川省人民医院 [4]成都市第三人民医院

出　　处：《中国超声医学杂志》2005年第10期794-796,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家"九五"科技重点攻关资助项目(No.96-905-02-01);国家自然科学基金资助项目(No.39770841)

摘　　要：目的研究高强度聚焦超声(HIFU)治疗乳腺癌后原发灶及腋窝阳性淋巴结中癌细胞表达免疫抑制因子血管内皮生长因子(VEGF)、转化生长因子-β1(TGF-β1)及转化生长因子-β2(TGF-β2)的变化.方法 HIFU组23例患者改良根治术前1～2周接受HIFU治疗,对照组25例仅行改良根治术,术后用免疫组化SP法检测两组原发灶及腋窝阳性淋巴结中VEGF、TGF-β1及TGF-β2的表达.结果原发灶局部,HIFU组三者的阳性率(VEGF,P＜0.05)及阳性肿瘤细胞数(三者均P＜0.05)较对照组低;腋窝阳性淋巴结中,HIFU组三者的阳性率(TGF-β2,P＜0.05)及阳性肿瘤细胞数(VEGF、TGF-β1,P＜0.05)也较对照组低.结论 HIFU治疗可以减少乳腺癌患者局部的免疫抑制因素,有助于改善局部免疫功能抑制状态.Objective To investigate the effects of high intensity focused ultrasound （HIFU） treatment on the change of immunosuppressive factors vascular endothelial growth factor （VEGF）, transforming growth factor-β1 （TGF-β1） and transforming growth factor-β2（TGF-β2） of breast cancer. Methods 23 patients with breast cancer underwent HIFU treatment for one to two weeks before modified radical mastectomy （HIFU group）, 25 patients only underwent modified radical mastectomy （control group） . After operation, SP immunohistochemical stain was used to detect the tumor-origin VEGF, TGF-β1 and TGF-β2 in primary tumor and in metastasis-positive axillary lymph nodes （HIFU, n=11, Control, n=12）, respectively. Results （1） In primary tumor, the positive rate of VEGF, TGF- β1 and TGF-β2 was 72%, 72% and 80% in control group, respectively while in HIFU group it was 30.4% （P〈 0.05）, 56. 5% and 69.6%, respectively. The number of positive tumor cells was 344.5±90.6/mm^2, 355.7±111.4/ mm^2 and 391.5±87.3/mm^2 in control group, respectively, while in HIFU group it was 235.9±93.8/mm^2, 243.7± 69.2/mm^2 and 209.8±77.1/mm^2, respectively （P〈0.05）. （2） In metastasis-positive axillary lymph nodes, the positive rate was 75%, 75% and 75% in control group, respectively, while in HIFU group it was 72.7%, 63.6% and 45.5% （P〈0.05）, respectively. The number of positive tumor cells was 410. 6±36.9/mm^2, 324.0±43.6/mm^2 and 366.4±130. 2/mm^2in control group, respectively, while in HIFU group, it was 315.3±51.4/mm^2 （P〈0.05）, 243.1± 52.0/mm^2 （P〈0. 05） and 249.1± 102.7/mm^2, respectively. Conclusions HIFU treatment could reduce local immunosuppressive factors, and would be helpful to reverse tumor-induced immunosuppression.

关 键 词：高强度聚焦超声 超声治疗 乳腺癌 基因表达 免疫抑制因子 

分 类 号：R737.9[医药卫生—肿瘤] R730.5[医药卫生—临床医学]