CCl_4肝损伤与免疫性肝损伤小鼠模型间差异性比较研究  

COMPARING STUDIES ON THE DIFFERENCES OF CCl_4 LIVER INJURY AND IMMUNITY LIVER INJURY MICE MODELS

作　　者：陈勇[1] 程明[1] 韩凤梅[1] 杜鹏[1] 

机构地区：[1]湖北大学中药生物技术省重点实验室,武汉430062

出　　处：《实验生物学报》2005年第5期417-422,共6页Acta Biologiae Experimentalis Sinica

基　　金：国家重大基础研究前期专项(2002ccc00300)湖北省科技厅重点攻关项目(2003AA303B02)武汉市科技局重点攻关项目(20036003042)

摘　　要：本文对比研究了CCl_4肝损伤与免疫性肝损伤小鼠模型肝组织病理切片、生理、生化指标及差异基因表达谱的变化,根据两种肝损伤模型的差异表达基因,初步探讨了它们致肝损伤的机理。结果表明,两组模型小鼠的ALT、AST等多项生化指标与正常小鼠相比存在显著性差异;通过表达谱芯片实验,在CCl_4组和免疫组中分别筛选得到379条和293条与正常组差异表达基因;其中有105 条基因在两个模型组中均差异表达(表达下调基因58条,表达上调基因47条)。提示CCl_4肝损伤与免疫性肝损伤小鼠模型间有相似性,但差异亦较明显。Comparison between CCl4 liver injury model and immunity liver injury model was studied through physiological, biochemical and gene expression profile methods, and the pathological mechanism of them were discussed. There are significant changes about the physiological, biochemical parameters including ALT,AST between two model groups and normal control group. Among the 14112 target genes, 379 and 293 differentially expressed genes were screened out from the both two model groups respectively in gene expression profile experiments, including 105 common, differentially expressed genes. The results indicate that the two models of CCl4 and immunity have some similarities. However, the differences between them are also obvious. It is useful for the further investigation on the liver-injury mechanism and pathological process of the two different liver-injury models.

关 键 词：小鼠 CCL4肝损伤 免疫性肝损伤 基因表达谱 基因芯片 小鼠模型 CCL4 肝损伤 免疫性 比较研究 

分 类 号：R575[医药卫生—消化系统]