辐射对小鼠精子发生过程中印记基因表达的影响  

Effects of ionizing radiation on imprinted gene expression in mouse spermatogenesis

作　　者：朱斌[1] 鹿亚超[2] 陈颖[2] 黄健 黄兴华[4] 赵经涌[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,苏州215007 [2]苏州大学放射医学与公共卫生学院医学遗传研究室,苏州215007 [3]人类基因组南方中心 [4]复旦大学遗传研究所

出　　处：《中华放射医学与防护杂志》2005年第5期426-428,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国防科工委资助项目(Y5573362)

摘　　要：目的筛选在小鼠精子发生过程中受电离辐射影响表达发生改变的印记基因。方法选择雄性BALBc小鼠8只,分为实验组和对照组X射线全身照射0.1Gyd,连续13d,建立辐射干扰小鼠精子发生模型。提取实验组和对照组睾丸RNA,采用上海生物芯片中心小鼠寡核苷酸基因表达谱芯片筛选表达发生改变的印记基因,对感兴趣的基因经半定量RTPCR验证。结果12个表达发生改变的印记基因,6个上调,6个下调,其中Igf2、Peg3基因Ratio值分别为3.859和0.397,经半定量RTPCR验证与芯片结果相符。结论X射线全身照射可导致小鼠睾丸部分印记基因表达发生改变。Objective To screen the imprinted gene with changed expression level by ionizing radiation in mouse spermatogenesis. Methods A mouse model, whose spermatogenesis was interfered by irradiation, was founded. A total of 8 male BALB/c mice were chosen and subjected to daily whole-body irradiation of 0.1 Gy Xrays for 13 days .Testis RNA from irradiated and control, mice was extracted, the imprinted genes expressing variably were screened with mouse oligo gene expression chip supplied by Shanghai Biochip Center and the interested genes were verified by semiquantitative RT-PCR. Results Altogether 12 imprinted genes were found,6 of them were up-regulated,and another 6 down-regulated, in which the ratio scores of Igf2 and Peg3 were 3.859 and 0. 397, respectively.The results confirmed by semlquantitative RT-PCR were consistent with those of gene chip. Conclusion The expression of some imprinted genes in mouse testis might be changed by chronic X-ray whole body irradiation.

关 键 词：小鼠 精子 印记基因 基因表达 放射损伤 睾丸 X射线 

分 类 号：Q691[生物学—生物物理学]