粘着斑激酶在TF/Ⅶa介导的乳腺癌细胞粘附、迁移中的作用  

The role of focal adhesion kinase in the breast carcinoma 's adhesion and migration mediated by the coagulation factor Ⅶa/tissue factor

作　　者：郭艳丽[1] 魏文宁[1] 宋善俊[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血研所,湖北武汉430022

出　　处：《西安交通大学学报（医学版）》2005年第5期467-469,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的研究粘着斑激酶(FAK)磷酸化在凝血因子Ⅶa与组织因子(TF)结合引起的乳腺癌细胞粘附、迁移中的作用,探讨TF促肿瘤转移的机制。方法用比色法测定凝血因子Ⅶa与高表达TF的乳腺癌MDA-MB231细胞的粘附数目;采用改良Bodyen小室检测肿瘤细胞迁移;免疫沉淀和Western blot方法检测FAK及其磷酸化。结果凝血因子Ⅶa与TF结合引起FAK磷酸化,参与肿瘤细胞迁移运动。结论FAK是TF介导的乳腺癌细胞粘附、迁移中的重要信号分子。Objective To study the role of focal adhesion kinase in the breast carcinoma cell adhesion and migration mediated by coagulation factor Ⅶ a combined with tissue factor to explore the mechanism of tissue factor promoting metastasis. Methods The count of adherent cells were detected by using colorimetric assay, cell migration was detected with modified Bodyen chamber, and the FAK and the tyrosine phosphorylation of FAK were detected by immunoprecipitation and Western blot. Results Factor Ⅶa combinaed with tissue factor induced the tyrosine phosphorylation of FAK, which took part in the cancer cell＇s migration. Conclusion FAK is an important message of the breast carcinoma＇s adhesion and migration mediated by TF.

关 键 词：粘着斑激酶 组织因子 粘附 迁移 

分 类 号：R730[医药卫生—肿瘤]