DLC血小板黏附特性与其表面能量的灰色关联分析  

Analysis of Grey Correlation between Surface Energy and Adhesive Characteristic of Platelet for DLC

作　　者：李伯刚[1] 殷杰[1] 那娟娟[1] 尹光福[1] 郑昌琼[1] 

机构地区：[1]四川大学材料科学与工程学院,成都610065

出　　处：《生物医学工程学杂志》2005年第1期20-24,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (3 9870 2 3 2 );教育部博士点基金资助项目 (2 0 0 0 610 0 4)

摘　　要：以不同工艺条件制备的 DL C材料为样本 ,进行了血小板黏附实验 ,测定了乙醇、水和不同组分比乙醇 /水溶液在各样本表面的平衡接触角 ,由此计算出表面张力、临界表面张力、界面张力、粘黏功 4个表面能量参数及表面张力的色散和极性分量 ,通过 T型关联度计算 ,分析了各参数对血小板黏附量和形态指数的影响。结果显示 :(1) 4个表面能量参数均与血小板的黏附量呈正相关 ,而形态指数则与表面张力、、界面张力和黏附功呈负相关 ;(2 )血小板的黏附量和形态指数与表面张力色散、极性分量的关联度异号 ,极性分量对血小板的黏附有利 ,而色散分量则于血小板的变形多功 ;(3)临界表面张力与血小板的黏附量和形态指数均有较大的正关联度 ;(4)表面张力极性分量对血小板黏附量和形态指数的影响与表面张力、界面张力和黏附功步调一致。由此得出 2个重要结论 :(1)血小板在 DL C表面的黏附特性与其表面界面性能密切相关 ,DL C的血液相容性取决于其亲水性和有限润湿的平衡 ,存在一个以临界表面张力为指标的血液相容性区域 ;(2 )血小板在 DL C表面的黏附和变形具有不同的能量机制 :亲水性的表面有利于血小板黏附 ,而黏附血小板的变形则要借助于表面的疏水作用。In this study, the diamond like carbon （DLC）samples made from different processes and processing conditions, were subjected to the test of platelet adhesion. The surface energy parameters such as surface tension, critical surface tension, interface tension, adhesive power, and polar branch and chromatic dispersion branch of surface tension based on determining balanced contact angle of ethanol, water and different ratios of ethanol/water solution on surfaces of the samples were calculated, respectively. Then the effects of these parameters on the amounts and deformation index of the platelets adhering to the samples were assessed by analyzing the T-type correlation degree in the Grey system. The results showed： （1） all degrees of correlation between surface energy parameters and adhesion amounts of platelet are positive, but for deformation index,the correlation degrees are negative except for critical surface tension; （2） the adhesion amounts of platelet increase with the rising polar branch of surface tension, while the deformation index increases with rising chromatic dispersion branch of surface tension; （3）Both adhesion amounts and deformation index of platelet are positively correlated with critical surface tension to a higher degrees; （4） the effects of polar branch of surface tension on adhesion amounts and deformation index of platelet keep pace with the surface tension, interface tension and adhesive power. Thus two important conclusions have been obtained ： （1） the adhesive characteristic of platelets to the surface of DLC is closely related with the surface energy of DLC; the hemocompatibility of DLC is decided by the balance between the polarity of DLC surface and the limited humidifying water on the surface ;there is a blood compatible range delimited by critical surface tension; （2） adhesion and deformation of platelets on surface of DLC have different energy mechanism： polar surface is advantageous to the adhesion, while the deformation is achieved with the aid of

关 键 词：血小板 黏附特性 表面能量 灰色关联 类金刚石薄膜 抗凝血性能 

分 类 号：R318.08[医药卫生—生物医学工程]