人类多形性胶质母细胞瘤中变异型IκBα基因抑制血管新生的研究  

Mutant-type IκBα gene inhibits angiogenesis in human glioblastoma multiform cells

作　　者：吴建梁[1] 扈玉华[1] 马树成[1] 张庆九[1] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000

出　　处：《中华实验外科杂志》2005年第11期1398-1399,i0006,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(30440016)

摘　　要：目的研究变异型IκBα(IκBαM)基因在人类多形性胶质母细胞瘤(GBM)中对血管新生的抑制作用。方法选用IκBαM基因转染的胶质瘤细胞株,通过制备血管新生及异位肿瘤生成两组动物模型,检测新生血管的数量及肿瘤中血管生长因子VEGF及IL-8的表达,分析IκBαM对肿瘤中血管新生的作用。结果IκBαM基因转染的人类GBM细胞中,新生血管数量显著减少,诱导形成的异位胶质瘤中的VEGF及IL-8的表达量也显著降低。结论IκBαM基因可有效抑制胶质瘤中的血管新生从而抑制肿瘤的生长,这可作为人类多形性胶质母细胞瘤抗血管治疗的理论基础。Objective To investigate the inhibitory effects of mutant-type IκBα （IgBaM） gene on angiogenesis in human glioblastoma multiform （GBM） cells. Methods IκBαM expressing cells were used to generate tumors in vivo. The effects of IκBαM on the VEGF and IL-8 in human GBM cells were compared by immunohistochemistry. To provide a more detail, a dorsal air sac method was used to quantitatively analyze the effeeets of IκBαM gene on tumor angiogenesis. Results Injection of cells treated with IκBαM resulted in a visible decrease in neovascularity and the decreased levels of VEGF and IL-8 expression were found in these cells compared with control. Conclusion IκBαM gene transfer could inhibit in vivo angiogenesis, therefore, suppress tumorigenicity of human glioma cells. This could be a theoretical basis in the anti-vascular therapy of human glioma.

关 键 词：胶质瘤 血管新生 VEGF 多形性胶质母细胞瘤 IΚBΑM 基因抑制 变异型 人类 胶质瘤细胞株 血管生长因子 

分 类 号：R73-3[医药卫生—肿瘤]