AML1基因转染对M-CSF受体基因的转录调节作用  

The effects of different transcripts of AML1 on the transactivation of M-CSF-R gene

作　　者：张青[1] 王敏[1] 邢海燕[1] 饶青[1] 王建祥[1] 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所,血液病医院,天津300020

出　　处：《中华血液学杂志》2005年第11期641-644,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目(30370593);人事部留学回国人员科技活动择优资助项目

摘　　要：目的观察AML1B基因及其异构体AML1A对靶基因启动子转录活性的影响,探讨造血干细胞定向分化和白血病发生的机制。方法构建AML1A和AML1B的表达质粒,与含有靶基因巨噬细胞集落刺激因子受体(M-CSF-R)启动子的荧光素酶报告质粒共转染非洲绿猴肾CV-1细胞,测定荧光素酶活性,分析AML1A及AML1B对靶基因转录活性的影响。结果AML1B对M-CSF-R具有明显的转录激活作用,这种作用具有明显的序列特异性和剂量依赖性;AML1A无转录激活作用,而且拮抗AML1B,使M-CSF-R的表达水平明显下降。结论AML1转录本结构的完整性对M-CSF-R的转录激活是必需的。AML1A可以干扰AML1B的转录激活作用。Objective To observe the effects of AML1A and AML1B, two splicing isoforms of AML1, on the transactivation of macrophage colony-stimulating factor receptor（M-CSF-R） , and explore the mechanism of hematopoietic stem cell committed differentiation and leukemogenesis. Methods The expressive plasmids of AML1A and AML1 B were constructed, and co-transfected into CV-1 cells with a luciferase reporter plasmid containing M-CSF-R promoter. The transactivity of M-CSF-R promoter was assayed by luminometer, Results AML1B exhibited a distinct transactivity to M-CSF-R promoter with a sequence-specificity and dosage-dependant manner. AML1A showed no any transactivity but antagonized the effect of AML1B, causing marked reduction of M-CSF-R expression. Conclusion An intact structure of AML1 is necessary for transactivation of M-CSF-R. AMLI A may interfere with the transactivation of AMLI B, and play a key role in the fine regulation of committed differentiation of hematopoietic cell.

关 键 词：AML1基因 基因转染 M-CSF受体基因 基因转录 造血干细胞 白血病 

分 类 号：R733.7[医药卫生—肿瘤]