乙酰丙酸和前体物对Rhodobacter sphaeroides 5-氨基乙酰丙酸合成的影响  

EFFECT OF LEVULINIC ACID AND PRECURSORS ON SYNTHESIS OF 5-AMINOLEVULINIC ACID IN RHODOBACTER SPHAEROIDES

作　　者：王俊卿[1] 张肇铭[1] 

机构地区：[1]山西大学生命科学与技术学院,太原030006

出　　处：《应用与环境生物学报》2005年第5期611-613,共3页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技部攻关项目(2001BA540C)资助~~

摘　　要：球形红细菌Rhodobacter sphaeroides光合成培养过程中(接种量2g/L),分别以不同方式加入不同浓度的乙酰丙酸(LA)、前体物(甘氨酸、琥珀酸),发现LA(30mmol/L)分3次加入,甘氨酸(60mmol/L)分2次加入,可使5-氨基乙酰丙酸合成酶(ALAS)的活性(149.1U/mg)提高,而其脱水酶(ALAD)活性(143.4U/mg)降低,使ALA产量提高到33.8mmol/L.In the photosynthetic culture of Rhodobacter sphaeroides with a suitable inoculation （2g/L）, levulinic acid （LA） and precursors, glycine and succinate, were added with different concentrations. When levulinic acid （30 mmol/L） was added three times, plus the twice additions of glycine （60 mmol/L）, significant enhancement of A1A production was observed. This phenomenon seemed to depend on low activity of ALAD（ 143.4 U/mg） , which catalyzed the synthesis of tetrapyrroles from ALA and high ALA synthesis activity （ 149.1 U/mg）, with release of suppression by twice additions of glycine. Finally ALA production increased up to 33.8 mmol/L. Fig 2, Ref 9

关 键 词：RHODOBACTER SPHAEROIDES 5-氨基乙酰丙酸 乙酰丙酸 甘氨酸 

分 类 号：TQ225.1[化学工程—有机化工]