克拉维酸产生菌的改良  

IMPROVEMENT OF CLAVULANIC ACID-PRODUCING STREPTOMYCES CLAVULIGERUS

作　　者：孟勇[1] 王忠彦[2] 官家发[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]四川大学生命科学学院,成都610064

出　　处：《应用与环境生物学报》2005年第5期614-617,共4页Chinese Journal of Applied and Environmental Biology

基　　金：四川省科技厅重点科技项目(02SG011-050);珠海联邦制药资助项目~~

摘　　要：以棒状链霉菌Streptomyces clavu ligerus CCRC11518(ATCC27064)为出发菌,采用经典物理和化学诱变剂处理的微生物诱变技术和现代理性化筛选方法,通过筛选抗终产物结构类似物舒巴坦钠突变株、底物甘油耐受型突变株、营养缺陷型突变株,最后获得一株克拉维酸高产突变株III50,其克拉维酸摇瓶产量为834.8μg/mL,是出发菌株产量(282.4μg/mL)的2.96倍.该突变株在琼脂斜面培养基上连续转接传代8代,克拉维酸的产量保持稳定.By means of traditional mutation method, Streptomyces clavuligerus CCRC11518 （ATCC27064） was used as parent strain, and Subtam resistant mark, glycerol tolerant mark and auxotrophic mark were added step by step. Finally, auxotrophic mutant Ⅲ50 with Subtam resistant genetic mark and glycerol tolerant genetic mark was obtained. The auxotrophic mutant Ⅲ50 had a clavulanic acid productivity of 834. 8 μg/mL, which was increased by 296% compared with the parent strain CCRC11518. This mutant had a stable productivity of clavulanic acid after reproducing 8 generations on the agar medium slant. Tab 12, Ref 8

关 键 词：克拉维酸 棒状链霉菌 菌种改良 理性化筛选 

分 类 号：TQ922[轻工技术与工程—发酵工程]