骨关节炎Ⅰ号方预防氢化可的松对兔软骨细胞的损伤作用(英文)  

Preventive effect of No.Ⅰrecipe for osteoarthritis on chondrocyte injury by hydrocortisone in rabbits

作　　者：乐宇民[1] 杜建[2] 

机构地区：[1]福建省第二人民医院风湿免疫科,福建省福州市350003 [2]福建中医学院,福建省福州市350001

出　　处：《中国临床康复》2005年第38期190-192,F0003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:骨关节炎Ⅰ号方中的补肾活血祛风湿中药具有治疗骨关节炎、预防糖皮质激素副作用的作用。目的:建立体外兔关节软骨细胞培养体系,观察骨关节炎Ⅰ号方预防氢化可的松对软骨细胞的损伤作用。设计:完全随机设计,对照实验。单位:福建省第二人民医院风湿免疫科。材料:实验于2003-11/2004-03在福建中医学院骨伤系分子实验室完成。关节炎Ⅰ号方由仙灵脾、巴戟天、川芎、三七、祖师麻组成。用胶原酶Ⅱ消化兔关节软骨,建立软骨细胞体外培养体系,在软骨细胞培养体系中分别加入不同浓度的骨关节炎Ⅰ号方与氢化可的松的混合液,根据加入的药物浓度不同随机分成2组,一组加入1.25g/L氢化可的松和/或150g/L骨关节炎Ⅰ号方,另一组加入2.50g/L氢化可的松和/或300g/L骨关节炎Ⅰ号方。每组再分为4个亚组,各组再分别分为对照组、氢化可的松组、氢化可的松+关节炎Ⅰ号方组、关节炎Ⅰ号方组,每组5孔。方法:①1.25g/L氢化可的松+150g/L关节炎Ⅰ号方组:每孔分别加入1.25g/L氢化可的松和150g/L骨关节炎Ⅰ号方混合液0.1mL。②1.25g/L氢化可的松组:每孔分别加入磷酸盐缓冲液和1.25g/L氢化可的松的混合液0.1mL。③150g/L骨关节炎Ⅰ号方组:每孔分别加入150g/L骨关节炎Ⅰ号方药液0.1mL。④对照组1:每孔分别加入磷酸盐缓冲液0.1mL。⑤2.50g/L氢化可的松+300g/L骨关节炎Ⅰ号方组:每孔分别加入2.50g/L氢化可的松和300g/L骨关节炎Ⅰ号方的混合液0.1mL。⑥2.50g/L氢化可的松组:每孔分别加入磷酸盐缓冲液和2.50g/L氢化可的松的混合液0.1mL。⑦300g/L骨关节炎Ⅰ号方组:每孔分别加入300g/L骨关节炎Ⅰ号方药液0.1mL。⑧对照组2:每孔分别加入磷酸盐缓冲液0.1mL。⑨上述各组加药培养24h后,用倒置显微镜观察各组软骨细胞形态变化。⑩采用四氮唑蓝比色法检测各孔软骨细胞的A值,以反映软骨细胞增BACKGROUND：Kidney-nourishing and blood activating Chinese herbs in the No.Ⅰ recipe for osteoarthritis have the effects of treating osteoarthritis and preventing the side effect of glucocorticoid. OBJECTIVE： To build up rabbit chondrocyte culture system in vitro to investigate the effect of preventive effect of No. I recipe for osteoarthritis on chondrocyte injury by hydrocortisone. DESIGN：A complete randomized design and controlled trial. SETTING： Department of Rheumatology and Immunology, Second Hospital of Fujian Province MATERIALS：The experiment was conducted from November 2003 to March 2004 at the Molecular Laboratory of Department of Orthopedics ＆ Grammatology, Fujian College of Traditional Chinese Medicine. No. I recipe for osteoarthritis consisted of Herba Epimedii, Radix Morindae Officinalis, Rhizoma Ligustic Chuanxiong, Radix Notoginseng and Daphne geraldii Nitsche. Articular cartilage was digested with collagenase Ⅱ , chondrocyte culture system in vitro was built up, then in the culture system the different concentrations of the mixture of No. Ⅰ recipe for osteoarthritis and hydroeortisone were added, according to the concentrations they were at random divided into two groups. One group was added 1.25 g/L hydrocortisone and/or 150 g/L No. Ⅰ recipe for osteoarthritis, the other group was added 2.50 g/L hydrocortisone and/or 300 g/L No. Ⅰ recipe for osteoarthritis. Each group was again subdivided as control, hydrocortisone, hydrocortisone ＋ No. Ⅰ recipe for osteoarthritis and No. Ⅰ recipe for osteoarthritis groups, with 5 wells in each subgroup. METHODS：①1.25 g/L hydrocortisone ＋150 g/L No. Ⅰ recipe for osteoarthritis group： In each well 0.1 mL mixture of 1.25 g/L hydrocortisone and 150 g/L No. I recipe for osteoarthritis was added. ②1.25 g/L hydrocortisone group： In each well 0.1 mL mixture of phosphate buffer and 1.25 g/L hydrocortisone was added. ③150 g/L No. Ⅰ recipe for esteoarthritis group： In each well No. Ⅰ recipe for osteoarthritis 0.1mL was added

关 键 词：骨关节炎 软骨细胞 氢化可的松 

分 类 号：R73[医药卫生—肿瘤]