胃癌细胞SGC-7901中乙酰肝素酶基因和CD44v6表达的关系  

Relationship between the CD44v6 expression and heparanase genein gastric carcinoma cell line SGC-7901

作　　者：周世庆[1] 汪素文[1] 朱靖宇[1] 张淑红[1] 真岩波[1] 张伯新[1] 

机构地区：[1]山东大学临床医学院济南市中心医院消化内科,山东济南250013

出　　处：《山东大学学报（医学版）》2005年第9期803-806,共4页Journal of Shandong University:Health Sciences

摘　　要：目的:探讨乙酰肝素酶(HPA)反义寡核苷酸(AS-ODN)对CD44v6表达的影响。方法:采用脂质体介导HPAAS-ODN转染人胃癌细胞株SGC-7901,RT-PCR检测HPAmRNA的表达,采用免疫细胞化学SABC法观察细胞内CD44v6表达的变化。结果:脂质体介导转染HPAAS-ODN48h后MSGC-7901胃癌细胞内HPAmRNA表达下降MCD44v6阳性表达率转染前69.4%M转染不同浓度的AS-ODN后分别为56.5%,54.1%,46.5%和41.3%。转染后细胞CD44v6表达明显减少(P<0.01)。结论:HPAAS-ODN可以有效抑制胃癌细胞CD44v6的表达。Objective： To evaluate the effect of heparanase antisense oligodeoxynucleotide（ASOND） on the expression of matrix metalloproteinase-2 （MMP-2）and tissue inhibitor of metalloproteinase-2 （TIMP-2）in human gastric carcinoma cell line SGC-7901. Methods： Human gastric adenocarcinoma cell line SGC-7901 was transfected with liposome-mediated heparanase AS-OND. The expression of heparanase mRNA was detected after transfection by semi-quantitative total RNAs RT-PCR. The changes of MMP-2 and TIMP-2 were observed by immunocytochemistry. Results： The expressions of heparanase mRNA and MMP-2 of SGC-7901 cells treated with AS-ODN of different final concentrations were significantly decreased 48 hours later compared with those of the controls （P〈0.01）. Conclusion： Heparanase AS-ODN has a significant inhibitory effect on the expression of MMP-2 in human gastric carcinoma cell line SGC-7901 .

关 键 词：乙酰肝素酶 基质金属蛋白酶-2 组织金属蛋白酶抑制剂-2 寡核苷酸类 反义 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]