腺苷三磷酸-氯化镁对新生大鼠缺氧缺血性脑损伤的干预作用  

Interventional effects of adenosine triphosphate-magnesium chloride in newborn rats with cerebral injury due to hypoxia-ischemia

作　　者：金正勇[1] 池永学[1] 许春花[1] 牛延军[1] 

机构地区：[1]延边大学医学院附属医院儿科,吉林省延吉市133000

出　　处：《中国临床康复》2005年第37期52-53,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨腺苷三磷酸-氯化镁对新生大鼠缺氧缺血脑损伤脑细胞凋亡的影响。方法:实验于2003-03/08在延边医学院儿科实验室完成。选用7d龄Wistar大鼠75只随机分为5组,即正常对照组15只,缺氧缺血性脑损伤组15只,生理盐水组15只,腺苷三磷酸治疗组15只,腺苷三磷酸-氯化镁治疗组15只,制备缺氧缺血性脑损伤模型腹腔内注射腺苷三磷酸及腺苷三磷酸-氯化镁,在缺氧缺血后72h麻醉状态下处死,取脑组织常规固定,切片行苏木精-伊红染色及原位缺口末端标记染色,测定脑细胞凋亡细胞数、凋亡百分率及凋亡面密度。结果:进入统计分析的大鼠为73只,模型组和腺苷三磷酸组各有1只大鼠在制作模型过程中死亡。①缺氧缺血性脑损伤组凋亡细胞数、凋亡百分率、凋亡面密度明显高于正常对照组[(34.24±3.96),(6.93±1.39)个/视野;(48.91±5.66)%,(9.90±1.98)%;(41.00±0.03)%,(7.93±0.0175)%,P<0.01]。②腺苷三磷酸组凋亡细胞数、凋亡百分率、凋亡面密度与缺氧缺血性脑损伤组及生理盐水组较接近(P>0.05)。③腺苷三磷酸-氯化镁凋亡细胞数、凋亡百分率、凋亡面密度[(16.27±2.55)个/视野,(23.24±3.64)%,(22.00±0.0262)%]明显低于缺氧缺血性脑损伤组、生理盐水组及腺苷三磷酸治疗组(P<0.01)。结论:腺苷三磷酸—氯化镁能通过血脑屏障,抑制脑细胞凋亡,对缺氧缺血脑细胞有保护作用。AIM：To study the effects of exogenous Adenosine triphosphate （ATP）-magnesium chloride on cell apoptosis in newborn rats after cerebral hypoxia-ischemia injury. METHODS：The experiment was carried out in the Laboratory of Podiatric Department,Yanbian Medical College from March to August 2003. Seventyfive Wistar rat, 7-day old, were selected and randomized into five groups： 15 rats in normal control group,15 in cerebral hypoxia-ischemia injury group（model group）, 15 in normal saline group, 15 in ATP group and 15 in ATP-magnesium chloride group.After modeling of cerebral hypoxia-ischemia injury, rats were undertaken intraperitoneal injection of ATP and ATP-magnesium chloride, and killed 72 hours after cerebral hypoxia-ischemia under anesthesia to take out brain tissues and make sections. Hematine-eosin staining and terminal deoxynucleotide transferase-modiated dUTP nick-end labeling were performed to calculate the amount of apoptutic cells, apoptusis percentage and apoptutic area. RESULTS： Seventy-three rats were involved in the statistical analysis, and one rat died during the modeling, respectively, in ATP group and ATP-magnesium chloride group. ①The number of apoptotic cells, apoptotic percentage, and apoptosis area were significantly higher in the model group than the normal control. [（34.24±3.96）, （6.93±1.39）per visual field; （48.91±5.66）%, （9.90±1.98）% ; （41.00±0.03）%, （7.93±0.0175）%,P〈0.01]. ②There were no differences in the number of apoptotic cells, apoptotic percentage and apoptosis area between the model group and normal saline group （P〉0.05）.③As compared with the other three groups, the number of apoptotic cells,apoptotic percentage, and apoptosis area were significantly lower in the ATP magnesium chloride group[（ 16.27±2.55）per visual field, （23.24±3.64）%,（22.00±0.0262）%, P〈0.01]. CONCLUSION：ATP-magnesium chloride can inhibit apoptosis of brain cells via the blood-cerebral barrier, and moreover, it can protect the brain cells

关 键 词：缺氧缺血 脑 氯化镁 腺苷三磷酸 动物 新生 细胞凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]