高压氧治疗大鼠短暂全脑缺血的最佳时间窗选择 以神经元特异性烯醇化酶活性变化为标准(英文)  

Optimal time window selection in hyperbaric oxygen treatment for rat transient global cerebral ischemia on the basis of neuron-specific enolase bioactivity changes

作　　者：刘天会[1] 陈瑞[2] 谭延国[3] 

机构地区：[1]首都医科大学附属北京友谊医院肝病中心,北京市100050 [2]首都医科大学生物化学与分子生物学系,北京市100050 [3]首都医科大学附属北京复兴医院检验科,北京市100050

出　　处：《中国临床康复》2005年第37期140-142,共3页Chinese Journal of Clinical Rehabilitation

基　　金：北京市教委科技发展计划基金资助项目(00KJ108)~~

摘　　要：背景:检测血中的神经元特异性烯醇化酶水平可以评估脑损伤的程度。目的:应用血浆神经元特异性烯醇化酶活性的动态变化来评估脑缺血后高压氧治疗效果的最佳时间窗。设计:析因设计。单位:首都医科大学生物化学与分子生物学系。材料:实验于2002-06在首都医科大学附属北京朝阳医院高压氧科实验室进行。取成年雌性SD大鼠54只,随机分成3组:假手术组(n=6),缺血再灌注组(n=24),高压氧组(n=24),后2组又分再灌注6,24,48及96h4个时相点,每个时相点6只大鼠。方法:①造模:以四动脉阻断法建立脑缺血再灌注动物模型,缺血20min后再灌注;假手术组同样手术,但不阻断动脉。②治疗:高压氧组大鼠行高压氧治疗(0.2MPa,吸纯氧45min),6h组在再灌注3h后行高压氧处理1次,其余3个时间点组大鼠在每天同一时间行高压氧处理,直至相应时间点取血。再灌注组和假手术组处于常压空气环境中。主要观察指标:高压氧组及再灌注组分别于再灌注6,24,48及96h取血,假手术组于再灌注24h取血。用酶联免疫吸附法测定血浆中的神经元特异性烯醇化酶活性。结果:经补充后54只大鼠进入结果分析。血浆神经元特异性烯醇化酶水平:假手术组为(1.97±0.09)μg/L;缺血再灌注组6,96h组高于假手术组[(2.80±0.26),(2.40±0.19)μg/L,P<0.05];高压氧组6h显著低于同时段缺血再灌注组[(2.04±0.27)μg/L,P<0.05],而24h治疗后略有升高,但差异没有显著性(P>0.05)。结论:缺血再灌注损伤导致血浆神经元特异性烯醇化酶水平升高,缺血再灌注组神经元特异性烯醇化酶水平出现6h和96h两次升高,可能与脑缺血过程中出现的急性神经元坏死及其后的迟发性神经元凋亡相关。高压氧治疗作用表现为神经元特异性烯醇化酶水平的恢复比同时相点缺血再灌注组快,以再灌注6h为高压氧最佳治疗时窗。BACKGROUND： The level of blood neuron-specific enolase may help predict the severity of brain damage. OBJECTIVE： To define the optimal time window of hyperbaric oxygen （HBO） treatment for brain ischemia based on the dynamical changes in plasma neuron-specific enolase bioactivity. DESIGN： Factorial design. SETTING： Department of Biochemistry and Molecular Biology of Capital University of Medical Sciences. PARTICIPANTS： The experiment was conducted in the Laboratory of the Department of Hyperbaric Oxygen, Beijing Cbaoyang Hospital Affiliated to Capital University of Medical Sciences in June 2002. Totally 54 adult female SD rats were randomized into 3 groups, namely sham operation group （n=6）, ischemia-reperfusion （IR） group （n=24）, and HBO group （n=24）, the latter 2 groups further divided into 4 groups according to the reperfusion time of 6, 24, 48 and 96 hours, with 6 rats in each subgroup. METHODS： ① Rat models of IR was prepared by occlusion of the 4 arteries for 20 minutes followed by reperfusion for different time. ② The rats in the sham operation received the same operation without blocking the arteries. The rats in HBO group were subjected to HBO treatment （0.2 MPa, pure oxygen for 45 minutes）, which was given after a 3-hour reperfusion in the 6-hour subgroup and scheduled once daily at the same time point in the other 3 subgroups until blood sampling. The rats in IR group and sham operation group were kept under normal pressure without additional oxygen. MAIN OUTCOME MEASURES： Blood samples were collected at the specified time points in IR and HBO groups and at 24 hours of reperfusion in the sham operation group. Enzyme-linked immunosorbent assay （ELISA） was used to determine the activity of plasma neuron-specific enolase. RESULTS： Totally 54 rats enter result analysis after supplementary. Plasma neuron-specific enolase level was significantly lower in the sham operation group （1.97±0.09）μg/L than in 6 and 96-hour subgroups in the IR group [（2.80±0.26

关 键 词：高压氧 脑缺血 再灌注 神经元特异性烯醇化酶 细胞凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]