聚合酶链反应联合Southern杂交检测急性非淋巴细胞白血病IgH重排基因  

DETECTIONOFIMMUNOGLOBULINHEAVYCHAIN(IgH)GENEREARRANGEMENTINANLLBYPOLYMERASECHAINREACTIONAMPLIFICATIONANDSOUTHERNBLOT

作　　者：徐兵[1] 周淑芸[1] 孙竟[1] 

机构地区：[1]广州第一军医大学南方医院

出　　处：《中华内科杂志》1996年第9期587-590,共4页Chinese Journal of Internal Medicine

基　　金：军队八五医药卫生科研基金

摘　　要：免疫球蛋白重链（ＩｇＨ）重排，可作为Ｂ淋巴细胞白血病的基因标志，为进一步了解急性非淋巴细胞白血病（急非淋）ＩｇＨ重排情况，用聚合酶链反应（ＰＣＲ）联合地高辛标记ＪＨ探针Ｓｏｕｔｈｅｒｎ杂交，检测４１例急非淋病人ＩｇＨＣＤＲ－Ⅲ区域。７例（１７．１％）ＰＣＲ扩增阳性，此７例阳性病例均经Ｓｏｕｔｈｅｒｎ杂交证实，本实验的灵敏度为１０－４～１０－５水平。１２例完全缓解病例中３例（２５．０％）重排阳性，此３例均于半年内临床复发。结果表明，ＩｇＨ重排并不局限于Ｂ淋巴系白血病，也可发生于急非淋病人，其机制可能是，部分急非淋起源于较原始的多向造血祖细胞或除髓系白血病克隆外。Immunoglobulinheavychain(IgH)generearangementservesasamarkerofclonalityinBlymphoproliferativemalignancies.InordertostudytheIgHrearangedgeneinacutenonlym-phoblasticleukemia(ANLL)patientswecombinepolymerasechainreaction(PCR)withSouthernblottodetect41ANLLpatientsand7ofthem(17.1%)werefoundtohaveIgHrearrangementbyPCRam-plification.Althese7positivecaseswereconfirmedbySouthernblot.Thesensitivityofthismethodwas10-4～10-5level.In12patientswithcompleteremission,3(25.0%)werefoundtohaveIgHrear-rangedgene.Althese3caseshadclinicalrelapsewithin6months.OurresultsshowthatIgHrear-rangementnotonlymayoccurinlymphoblasticleukemiaofBlineage,butalsocanbefoundinANLL.ThemechanismmaybethatinsomeANLLpatients,theleukemictransformingeventmightinvolvestemcelscapableofbothBcelandmyeloiddiferentiationorANLLmightdiferentiatealongdiferentlineagewithpredominantappearanceofoneortheothersubcloneinthecourseofthedisease.

关 键 词：聚合酶链反应 白血病 免疫球蛋白类 

分 类 号：R733.704[医药卫生—肿瘤]