黄色隐球酵母生产辅酶Q_(10)发酵条件的优化  

Optimization of Fermentation Conditions of Coenzyme Q_(10) Production by Cryptococcus luteolus

作　　者：叶博[1] 刘玲[1] 李革[2] 

机构地区：[1]沈阳农业大学食品学院 [2]大连水产学院食品工程系,辽宁大连116023

出　　处：《微生物学杂志》2005年第4期60-63,共4页Journal of Microbiology

摘　　要：从黄色隐球酵母L3302提取辅酶Q10,经过氮源、碳源、初始pH、发酵温度等的研究分析,得到最佳的发酵条件。通过最优化实验确定培养基:蔗糖和葡萄糖各1.25g/L;酵母膏和玉米浆各0.3g/L;pH值6.5,温度28℃,接种量5%,装液量为50mL/500mL;生长因子以蛋白水解液为优。按此发酵条件上罐发酵,得到菌体生长量为12.8g/L发酵液,辅酶Q10的产量为1.82mg/100mL发酵液。CoQ10 fermentation factors by Cryptococcus luteolus L3302 were studied. Orthogonal experiment showed that optimum conditions for CoQ10 production are 1.25 g/L of glucose, 1.25 g/L of sucrose, 0.3 g/L of yeast extract, 0.3 g/L of maize syrup, initial pH at 6.5, inoculation at 5% , temperature at 28℃ , and volume of medium was 50 mL/500 mL. Under this optimized conditions biomass of fermentor reached up to 12.8 g/L and CoQ10 concentration was 1.82 mg/100 mL.

关 键 词：辅酶Q10 发酵 优化 

分 类 号：Q93[生物学—微生物学]