急性非淋巴细胞白血病患儿人类端粒酶逆转录酶基因的表达  

Study of Human Telomerase Reverse Transcriptase Gene Expression in Acute Non - Lymphocytic Leukemia Children

作　　者：李熙鸿[1] 熊珍玉[1] 王晓阳[1] 汪凤兰[1] 刘柏林[2] 张燕[2] 

机构地区：[1]四川大学华西第二医院儿科,成都610041 [2]四川大学华西基础医学与法医学院分子生物学研究室,成都610041

出　　处：《实用儿科临床杂志》2005年第11期1112-1113,共2页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(39970836)四川省卫生厅科学研究基金项目资助(040037)

摘　　要：目的探讨人类端粒酶逆转录酶(hTERT)基因表达与儿童急性非淋巴细胞白血病(ANLL)关系和临床意义。方法采用逆转录-聚合酶链反应(RT-PCR)方法检测HL-60白血病细胞株、14例ANLL患儿和11例正常对照的hTERT基因表达。结果HL-60白血病细胞株、14例ANLL患儿中11例及健康儿童2例有hTFRT基因表达;hTERT基因表达见于ANLL所有亚型;其表达水平明显高于正常对照,但较K562细胞株低。结论儿童ANLLhTERT-mRNA表达水平上调;hTERT基因表达与儿童ANLL发生发展有关。Objective To explore the gene expression of human telomerase reverse transcriptase （hTERT）in childhood acute non-lymphocytic leukemia（ANLL） and its clinical implication. Methods Expression of hTERT mRNA was detected in HL- 60 leukemia cell line, 14 ANLL children and 11 healthy children by reverse transeriptase - polymerase chain reaction （RT-PCR）. Results hTERT gene was expressed in HL-60 cell line,ANLL children （11/14） and healthy children （2/11）. The expression of hTERT gene was observed in all subtypes of ANLL. Furthermore, there were statistically significant differences in expression levels of hTERT gene between children with ANLL and healthy children （ t = 5. 034 P = 0）. Conclusions The up - regulation of gene ex- pression of hTERT may play a very important role in the progression of children ANLL. The detection of hTERT expression may be a useful additional method for monitoring the state of illness.

关 键 词：白血病 非淋巴细胞 急性 人类端粒酶逆转录酶 儿童 

分 类 号：R733.7[医药卫生—肿瘤]