ClC-3在人胶质瘤中的表达及分布  

Expression and distribution of C1C-3 in human glioma specimen

作　　者：徐冰[1] 罗祺[2] 王心蕊[3] 刘潇[1] 王医术[3] 孙连坤[1] 

机构地区：[1]吉林大学基础医学院病理生理教研室,吉林长春130021 [2]吉林大学第一医院神经外科,吉林长春130021 [3]吉林大学基础医学院病理教研室,吉林长春130021

出　　处：《中国病理生理杂志》2005年第11期2188-2191,共4页Chinese Journal of Pathophysiology

基　　金：吉林大学创新基金资助项目(No.2003CX100)

摘　　要：目的探讨ClC-3在人脑胶质瘤中的表达及其生物学意义。方法应用免疫组织化学染色技术检测24例人胶质瘤以及4例脑转移癌手术标本中ClC-3蛋白的表达;RT-PCR检测ClC-3蛋白表达阳性的手术标本中其mRNA表达。结果4例正常脑组织ClC-3蛋白的表达为阴性;而蛋白表达阳性的19例胶质瘤及4例脑转移癌中,ClC-3主要位于瘤细胞和微血管内皮细胞的胞浆或胞膜上。蛋白表达阳性的手术标本中16例胶质瘤和4例脑转移癌检测到ClC-3mRNA的表达。少突胶质细胞瘤Ⅲ级中ClC-3的mRNA和蛋白表达明显高于其Ⅱ级。结论ClC-3在人胶质瘤及脑转移癌组织中普遍表达,并且可能与少突胶质细胞瘤病理分级相关。AIM： To investigate the expression of voltage - gated chloride channels （C1C） - 3 protein and mRNA in human ghoma specimen and its biological function. METHODS： The expressian of C1C- 3 was observed by immunohistochemical staining in 24 cases of human glioma, 4 eases of brain metastic cancer specimens and 5 cases of normal brain tissue as control ; The C1C- 3 mRNA expression were detected in the specimens with positive expression of C1C - 3 protein by RT - PCR. RESULTS： C1C - 3 protein was found negative in 4 eases of normal brain tissues and positive in 19 cases of human glioma and 4 cases of brain metastic cancer specimens. C1C - 3 protein was mainly expressed in the membrane or cytoplasm of neoplastic cells and microvaseular endothelial cells. The expression of C1C - 3 mRNA was detected in 16 cases of human glioma and 4 cases of brain metastasis cancer specimens among the tissues with the positive expression of C1C - 3 protein. The level of protein and RNA of C1C - 3 in high malignant oligodendrngliomas was higher than that in low malignant ones. CONCLUSION： C1C - 3 is generally expressed in human glioma and brain metastic cancer and is probably correlated with the classifieation of its pathological malignance.

关 键 词：神经胶质瘤 蛋白质C1C-3 免疫化学 氯化物通道 

分 类 号：R735.2[医药卫生—肿瘤]