COX-2抑制剂(NS-398)对结肠癌HT-29细胞体外侵袭力的作用及CD44v6、nm23-H1基因的调节  

Effect of NS-398 on invasion of colon cancer HT-29 cells in vitro and its regulation by CD44v6 and nm23-H1 genes

作　　者：贾晓青[1] 阎明[1] 孟繁立[1] 钟宁[1] 夏光涛[1] 李延青[1] 张尚忠[1] 

机构地区：[1]山东大学齐鲁医院消化科,山东济南250012

出　　处：《中国病理生理杂志》2005年第11期2197-2200,共4页Chinese Journal of Pathophysiology

摘　　要：目的研究NS-398对结肠癌HT-29细胞体外侵袭力的作用及CD44v6、nm23-H1基因的调节。方法通过流式细胞仪检测COX-2和CD44v6的表达,MTT检测细胞活性,改良的Boyden小室法观察HT-29细胞侵袭重组基底膜的能力,RT-PCR观察nm23-H1mRNA的表达。结果HT-29细胞COX-2表达阳性,0.1、1.0、10μmol/L NS-398可显著抑制HT-29细胞侵袭重组基底膜的能力,且上述作用与NS-398的毒性作用无关。NS-398可下调CD44v6的表达,上调nm23-H1mRNA的表达。结论NS-398具有抑制结肠癌细胞HT-29体外侵袭力的作用,下调CD44v6的表达和上调nm23-H1mRNA的表达可能是其作用机制。AIM： To study the anti - invasive effect of NS - 398 on colon cancer cell hne HT - 29 in vitro an its regulation by CD44v6 and nm23 - H1 genes. METHODS： Flow cytometry was used to detect the expression of COX - 2 and CD44v6 in HT - 29 cells. MTT was used for cell survival rate tests. The modified Boyden chamber model was used for quantitative invasion assay. RT - PCR was used to detect the expression of nm23 - H1 mRNA. RESULTS： Flow cytometry analysis showed that COX - 2 was positive in HT - 29 cells. NS - 398 had significant inhibitory effects on invasion of HT - 29 cells, which had no relation with its cytotoxicity. NS- 398 down - regulated the expression of CD44v6 and up - regulated the expression of nm23 - H1 mRNA. CONCLUSION： NS - 398 has an anti - invasive effect on HT - 29 cells in vitro. Down - regulation of CD44v6 and up - regulation of nm23 - H1 may be its underlying mechanisms.

关 键 词：HT-29细胞 NS-398 结肠肿瘤 侵袭 肿瘤 CD44V6 nm23-HI 前列腺内过氧化物合酶 

分 类 号：R735.35[医药卫生—肿瘤]