救脑宁注射液对培养神经细胞缺氧缺糖及再灌注损伤的拮抗作用  

Antagonistic action of Jiunaoning injection aganist oxygen/glucose-deprived and reperfusion injury-induced cultured rat cortical neurons

作　　者：王芳[1] 李克玲[1] 黄启福[1] 苏涛峰[1] 徐礼五[1] 

机构地区：[1]北京中医药大学病理教研室,北京100029

出　　处：《中国病理生理杂志》2005年第11期2253-2256,共4页Chinese Journal of Pathophysiology

摘　　要：目的观察救脑宁注射液对离体培养神经细胞缺氧缺糖和再灌注损伤的拮抗作用。方法将原代培养第9d的新生大鼠大脑皮层神经细胞进行缺氧缺糖及再灌处理,利用酶联免疫检测仪按MTT比色法观察了不同浓度(终浓度为0.5-5mL/L)的救脑宁注射液对培养神经细胞缺氧缺糖和再灌注不同时间损伤活细胞代谢的保护作用。结果救脑宁在所研究的浓度范围内对神经细胞的保护作用呈现出浓度依赖性;并且1.5-2.5mL/L浓度范围的救脑宁注射液能明显提高损伤神经细胞的活性和代谢率,减轻乳酸脱氢酶的漏出率。结论救脑宁注射液对缺氧缺糖损伤的离体培养神经细胞有一定的保护作用。AIM： To determine whether Jiunaoning injection has protective effects on oxygen/glucose- deprived and reperfusion injury- induced neurons. METHODS： Various concentrations of Jiunaoning injection （0.5- 5 mL/L） were used to observe their effects on cultured rat cortical neurons induced by oxygen/glucose - deprived and reperfusion injury in various time points. The neuronal metabolic rate and viability were assessed by using 3 - （4,5 - dimethyhhiazol） - 2, 5 - diphenyl - tetra zoli- umbromide （MTT） and lactate dehydrogenase （LDH） assay. RESULTS： Jiunaoning injection enhanced the neuronal metabolic rate in a dose - dependent manner in the range from 0.5 to 5 mL/L, and Jiunaoning injection （ 1.5 - 2.5 mL/L） enhanced the neuronal metabolic rate, decreased the cell death rate and depressed LDH leak rate significantly. CONCLUSION： Jiunaoning injection has an affirmative protective effect on oxygen/glucose- deprived and reperfusion- induced neuronal injury.

关 键 词：救脑宁注射液 再灌注损伤 神经元 大鼠 

分 类 号：R363[医药卫生—病理学]