NT-3基因修饰许旺细胞与神经干细胞联合移植促进大鼠全横断脊髓受损伤神经元的存活及其轴突再生  

Combinative grafting neural stem cells and NT-3 genetically modified Schwann cells to promote the injured neurons'survival and axonal regeneration of the transected spinal cord rat

作　　者：郭家松[1] 曾园山[2] 李海标[2] 黄文林[3] 陈穗君 

机构地区：[1]南方医科大学南校区解剖学教研室 [2]中山大学中山医学院组织胚胎学教研室神经科学研究室,广州市510080 [3]中山大学肿瘤防治中心

出　　处：《中华显微外科杂志》2005年第4期337-339,i0005,共4页Chinese Journal of Microsurgery

基　　金：国家自然科学基金(30270700);广东省社会发展攻关项目(2003C33808);高等学校博士学科点专项科研基金(2004558068)

摘　　要：目的探讨神经营养素-3基因修饰许旺细胞(NT-3-SCs)与神经干细胞(NSCs)联合移植对大鼠因脊髓全横断而受损伤的神经元存活及其轴突再生的作用。方法将NT-3-SCs及LacZ报告基因修饰SCs(LacZ-SCs)与NSCs联合移植到全横断性脊髓损伤处,60 d后在脊髓横断处尾侧注射荧光金(FG)进行逆行标记。第67天,取材进行组织学检测大脑皮质体感区、红核及脊髓横断处头侧FG标记神经元;大脑皮质体感区、红核和脊髓背核内存活的神经元以及脊髓损伤处再生的轴突。结果大脑皮质体感区、红核及脊髓L1背核内存活的神经元由多到少依次为NT-3-SCs与NSCs联合组、LacZ-SCs与NSCs联合组和实验对照组。NT-3-SCs与NSCs联合组和LacZ-SCs与NSCs联合组的大脑皮质体感区、红核和脊髓横断处头侧有FG标记神经元;脊髓横断处及其附近组织有5-HT、CGRP和SP阳性神经纤维。结论NT-3-SCs与NSCs联合移植能够促进脊髓全横断损伤后神经元的存活以及轴突再生。Objective To explore the effects of neurotrophin-3 genetically modified Schwann cells （NT-3-SCs） and neural stem cells （NSCs） combinative transplantation to promote the neurons＇ survival and axonal regeneration of the spinal cord transected rat. Methods After the transected spinal cord injury （SCI） model was established in SD rats, NT-3-SCs or LacZ report gene modified Scwhann cells（LacZ-SCs） were combinative transplanted with NSCs into the transected site. 60 days later, fluorogold（FG） was injected into the caudal spinal cord fo the transected site. 7days after FG injected, the sacrifice, cryosection and morphology serious studies were performed to observe the FG labeled neurons in the rostral spinal cord （RSC） of transected site, red nuclei（RN） and the sensorimotor cortex（SMC） ; The survival neurons in the L1 Clark＇ s nuclei（ CN）, RN and SMC were couut, and regenerated axons within or near the transected spinal cord observed. Results From more to less, the order of groups of the survival neurons in the CN, RN and SMC was NT-3-SCS ＆ NSCs group, LacZ-SCs ＆ NSCs group, experimental control group. In the NT-3-SCs ＆ NSCs group and LacZ-SCs ＆ NSCs group, there were 5-HT, CGRP and SP positive axons within or near the transected spinal cord. And some FG-labeled cells were found in RSC, RN and SMC. Conclusion Combinative grafting NSCs and NT-3-SCs could promote the neurons＇ survival and axonal regeneration of the spinal cord injured rat.

关 键 词：神经营养素-3 基因修饰 许旺细胞 神经干细胞 脊髓损伤 再生 全横断性脊髓损伤 NT-3基因修饰 神经元存活 联合移植 

分 类 号：R651.2[医药卫生—外科学] R329-33[医药卫生—临床医学]